de Rie M A, Schumacher T N, van Schijndel G M, van Lier R A, Miedema F
Central Laboratory, the Netherlands Red Cross Blood Transfusion Service, Amsterdam.
Cell Immunol. 1989 Feb;118(2):368-81. doi: 10.1016/0008-8749(89)90385-7.
Cluster of differentiation ([CD]) 19 antigens are B-cell-specific molecules expressed on virtually all human cells of the B-lymphocyte lineage except plasma cells. We produced a new anti-CD19 monoclonal antibody (McAb), CLB-CD19, that was used to study the role of CD19 molecules in B-cell activation. Anti-CD19 McAb induced mobilization of free intracellular calcium ([Ca2+]i) in Daudi cells, but not in normal spleen or tonsillar B cells, for which crosslinking with a second anti-mouse Ig antibody was not required. Anti-CD19 McAb inhibited B-cell proliferation induced by anti-IgM coupled to Sepharose beads. This inhibitory effect was overcome by the addition of nonmitogenic concentrations of phorbol myristate acetate. Anti-CD19 McAb did not interfere with Staphylococcus aureus- or B-cell growth factor-induced B-cell proliferation. Anti-CD19 McAb inhibited T-cell-dependent polyclonal B-cell differentiation in pokeweed mitogen-, interleukin 2-, or anti-CD3-driven culture systems. Delayed addition studies showed that once differentiation of B cells was induced, CD19 molecules lost their regulating function. Taken together, our results indicate that CD19 molecules play a regulatory role in B-cell proliferation and differentiation.
分化簇（[CD]）19抗原是B细胞特异性分子，除浆细胞外，几乎在B淋巴细胞谱系的所有人类细胞上均有表达。我们制备了一种新的抗CD19单克隆抗体（McAb），即CLB-CD19，用于研究CD19分子在B细胞活化中的作用。抗CD19 McAb可诱导Daudi细胞内游离钙（[Ca2+]i）的动员，但在正常脾脏或扁桃体B细胞中则不能，对此不需要用第二种抗小鼠Ig抗体进行交联。抗CD19 McAb可抑制与琼脂糖珠偶联的抗IgM诱导的B细胞增殖。添加非促有丝分裂浓度的佛波醇肉豆蔻酸酯可克服这种抑制作用。抗CD19 McAb不干扰金黄色葡萄球菌或B细胞生长因子诱导的B细胞增殖。抗CD19 McAb可抑制在商陆有丝分裂原、白细胞介素2或抗CD3驱动的培养系统中T细胞依赖性多克隆B细胞分化。延迟添加研究表明，一旦诱导B细胞分化，CD19分子就会失去其调节功能。综上所述，我们的结果表明CD19分子在B细胞增殖和分化中起调节作用。
