D'Alessandro A M, Stratta R J, Sollinger H W, Kalayoglu M, Pirsch J D, Belzer F O
Department of Surgery, University of Wisconsin School of Medicine, Madison.
Diabetes. 1989 Jan;38 Suppl 1:7-9. doi: 10.2337/diab.38.1.s7.
We have recently reported successful 72-h preservation of the canine pancreas with a new cold-storage solution developed at the University of Wisconsin (UW solution). Over 10 mo, we performed 11 combined pancreas-kidney and 4 isolated-pancreas transplants with this solution. In situ cooling of the donor pancreas was performed with 1000 ml of UW solution followed by ex vivo perfusion with an additional 250-500 ml. Graft preservation times ranged from 3 to 19 h (mean 10.2 h). Pancreas transplants were vascularized whole-organ grafts with pancreaticoduodenocystostomy. Early graft function was excellent as assessed by immediate insulin independence, high urinary amylase and low serum amylase levels, and a technetium perfusion index indicating good pancreatic blood flow. There were no episodes of primary nonfunction, graft pancreatitis, or vascular thrombosis. Actuarial patient and graft survival at 1 mo was 92.9%. We conclude that UW solution provides excellent early graft function for up to 19 h of cold storage. Based on previously reported data on its efficacy in liver and kidney preservation, UW solution seems ideally suited as a universal intra-aortic flush and cold-storage solution.
我们最近报告了使用威斯康星大学研发的一种新型冷藏溶液（UW溶液）成功保存犬胰腺72小时的情况。在10多个月的时间里，我们使用这种溶液进行了11例胰肾联合移植和4例单独胰腺移植。用1000毫升UW溶液对供体胰腺进行原位冷却，随后再用250 - 500毫升溶液进行体外灌注。移植物保存时间为3至19小时（平均10.2小时）。胰腺移植采用胰十二指肠囊肿造口术进行血管化全器官移植。通过立即实现胰岛素自主、高尿淀粉酶和低血清淀粉酶水平以及表明胰腺血流良好的锝灌注指数评估，早期移植物功能良好。没有原发性无功能、移植物胰腺炎或血管血栓形成的情况。1个月时患者和移植物的实际生存率为92.9%。我们得出结论，UW溶液在长达19小时的冷藏中能提供出色的早期移植物功能。基于先前报道的其在肝脏和肾脏保存方面的疗效数据，UW溶液似乎非常适合作为一种通用的主动脉内冲洗和冷藏溶液。
