Toffoli-Kadri Mônica Cristina, Carollo Carlos Alexandre, Lourenço Letícia Dias, Felipe Josyelen Lousada, Néspoli José Henrique Brandini, Wollf Luis Guilherme Campos, Resende Glenda Mara Sousa, de Lima Jaqueline Rodrigues, Franco Vanessa Natachi Penteado, Vieira Maria do Carmo, de Siqueira João Máximo
Laboratório de Biofisiofarmacologia, UFMS,CP 549, 79070-900, Campo Grande, MS, Brazil.
Laboratório de Farmacognosia, UFMS, Campo Grande, MS, Brazil.
J Ethnopharmacol. 2014 Apr 28;153(2):461-8. doi: 10.1016/j.jep.2014.03.008. Epub 2014 Mar 12.
Achyrocline alata is a locally marketed (Mato Grosso do Sul/ Brazil) herb used in folk medicine as an anti-inflammatory and a sedative. Evaluate the anti-inflammatory properties of Achyrocline alata in both in vivo and in vitro models.
A hydroethanolic extract from inflorescences of Achyrocline alata (HEAa) was characterized by HPLC-DAD and compared to standards (chlorogenic acid; isoquercetrin; quercetin; 4,2',4'-trihydroxy-6'-methoxychalcone; gnaphalin; 3-O-methyl-quercetin; 3,5-dicaffeoyl-quinic acid and 4,5-dicaffeoyl-quinic acid). The in vivo anti-inflammatory properties of the HEAa (4, 20 and 100 mg/kg, per os) were evaluated using the following animal models: carrageenan-induced paw edema in rats, carrageenan-induced vascular permeability and peritonitis in mice and an acetic acid-induced writhing model to test antihyperalgesic activity in mice. In vitro assays were performed to study the effects of the HEAa (0.16, 0.8 and 4 mg/ml) on the cell viability, cell spreading and production of NO and H2O2 in stimulated macrophages.
The A. alata extract inhibited the development of edema and vascular permeability, reduced polymorphonuclear cell recruitment in the acute peritonitis assay and decreased the amount of writhing induced by acetic acid. The HEAa did not increase NO/H2O2 production, while it did inhibit production when the macrophages were stimulated by LPS or PMA at all tested concentrations. In the presence of HEAa, macrophage spreading did not increase even after stimulation with LPS. Additionally, the HEAa was nontoxic to macrophages at all tested concentrations.
The HEAa displayed anti-inflammatory and antihyperalgesic effects, which supports the use of this plant in folk medicine. These effects might be due to the flavonoids and phenylpropanoids derivatives present in the HEAa.
翅果菊是一种在当地（巴西南马托格罗索州）市场销售的草药，在民间医学中用作抗炎药和镇静剂。评估翅果菊在体内和体外模型中的抗炎特性。
采用高效液相色谱-二极管阵列检测法（HPLC-DAD）对翅果菊花序的水乙醇提取物（HEAa）进行表征，并与标准品（绿原酸、异槲皮苷、槲皮素、4,2',4'-三羟基-6'-甲氧基查耳酮、鼠曲草素、3-O-甲基槲皮素、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸）进行比较。使用以下动物模型评估HEAa（4、20和100毫克/千克，口服）的体内抗炎特性：角叉菜胶诱导的大鼠足爪水肿、角叉菜胶诱导的小鼠血管通透性和腹膜炎，以及乙酸诱导的扭体模型以测试小鼠的抗痛觉过敏活性。进行体外试验以研究HEAa（0.16、0.8和4毫克/毫升）对刺激的巨噬细胞的细胞活力、细胞铺展以及一氧化氮（NO）和过氧化氢（H2O2）产生的影响。
翅果菊提取物抑制水肿的发展和血管通透性，在急性腹膜炎试验中减少多形核细胞募集，并减少乙酸诱导的扭体次数。HEAa不会增加NO/H2O2的产生，而在所有测试浓度下，当巨噬细胞受到脂多糖（LPS）或佛波酯（PMA）刺激时，它确实会抑制其产生。在存在HEAa的情况下，即使在用LPS刺激后，巨噬细胞铺展也不会增加。此外，在所有测试浓度下，HEAa对巨噬细胞无毒。
HEAa具有抗炎和抗痛觉过敏作用，这支持了这种植物在民间医学中的应用。这些作用可能归因于HEAa中存在的黄酮类和苯丙素类衍生物。
