Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida, Yucatán, México.
Laboratorio de Química Farmacéutica, Facultad de Química, Universidad Autónoma de Yucatán, Calle 43, No 613 x calle 90, Col. Inalámbrica, CP. 97069, Mérida, Yucatán, México.
Inflammopharmacology. 2021 Apr;29(2):513-524. doi: 10.1007/s10787-021-00795-x. Epub 2021 Mar 16.
The present paper sought to investigate the in vitro and in vivo anti-inflammatory effects of the methanolic extract (ME), hexane-ethyl acetate fraction E (FE) found in Chrysophyllum cainito fruits (CCF), as well the lupeol acetate (LA) obtained from FE on lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. The macrophages were treated with ME, FE or LA at various concentrations and the viability of cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide method. Production of pro-inflammatory (IL-1β, IL-6, and TNF-α) and anti-inflammatory (IL-10) cytokines, as well as the nitric oxide (NO) and hydrogen peroxide (HO) levels was determined using macrophages treated with ME, FE or LA at various concentrations and stimulated with LPS as an in vitro model. Afterwards, we evaluated the anti-inflammatory effects in vivo using the TPA-induced ear edema and carrageenan-induced paw edema tests in mice and production of inflammatory mediators was estimated in serum samples. The results showed that the ME, FE and LA from fruits, FE and LA were able to trigger an inhibition in NO and HO levels, as well as IL-1β, IL-6, and TNF-α released by macrophages in a concentration-dependent manner. LA from C. cainito fruits was found to significantly attenuate carrageenan-induced paw edema and TPA-induced ear edema. Therefore, the results suggest ME, FE and LA isolated from C. cainito fruits have anti-inflammatory effects on macrophages without affecting cell viability.
本研究旨在探讨番石榴果实甲醇提取物（ME）、正己烷-乙酸乙酯部分（FE）以及从 FE 中提取的羽扇醇乙酸酯（LA）的体外和体内抗炎作用。用不同浓度的 ME、FE 或 LA 处理脂多糖（LPS）刺激的小鼠腹腔巨噬细胞，采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法测定细胞活力。采用 LPS 刺激的体外模型，用不同浓度的 ME、FE 或 LA 处理巨噬细胞，测定促炎（IL-1β、IL-6 和 TNF-α）和抗炎（IL-10）细胞因子以及一氧化氮（NO）和过氧化氢（HO）水平。然后，我们在小鼠中评估了 TPA 诱导的耳肿胀和角叉菜胶诱导的足肿胀试验中的体内抗炎作用，并估计了血清样本中炎症介质的产生。结果表明，ME、FE 和 LA 能够抑制 NO 和 HO 水平的产生，以及巨噬细胞中 IL-1β、IL-6 和 TNF-α的释放，且呈浓度依赖性。番石榴果实中的 LA 显著减轻了角叉菜胶诱导的足肿胀和 TPA 诱导的耳肿胀。因此，研究结果表明，番石榴果实中分离得到的 ME、FE 和 LA 对巨噬细胞具有抗炎作用，而不影响细胞活力。
