Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Department of Chemical Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo-ku, Tokyo 112-8681, Japan.
FEBS Lett. 2014 May 2;588(9):1680-5. doi: 10.1016/j.febslet.2014.03.010. Epub 2014 Mar 13.
Phosphatidylglycerophosphate (PGP) synthase, encoded by PGP1 and PGP2 in Arabidopsis, catalyzes a committed step in the biosynthesis of phosphatidylglycerol (PG). In this study, we isolated a pgp1pgp2 double mutant of Arabidopsis to study the function of PG. In this mutant, embryo development was delayed and the majority of seeds did not germinate. Thylakoid membranes did not develop in plastids, mitochondrial membrane structures were abnormal in the mutant embryos, and radiolabeling of phospholipids showed that radioactivity was not significantly incorporated into PG. These results demonstrated that PG biosynthesis is essential for the development of embryos and normal membrane structures of chloroplasts and mitochondria.
磷脂酰甘油磷酸合酶（PGP）由拟南芥中的 PGP1 和 PGP2 编码，催化磷脂酰甘油（PG）生物合成中的一个关键步骤。在这项研究中，我们分离了拟南芥的 pgp1pgp2 双突变体，以研究 PG 的功能。在这个突变体中，胚胎发育延迟，大多数种子不能发芽。质体中的类囊体膜没有发育，突变体胚胎中的线粒体膜结构异常，磷脂的放射性标记显示放射性没有明显掺入 PG。这些结果表明，PG 生物合成对于胚胎发育和叶绿体及线粒体的正常膜结构是必不可少的。
