Shenzhen Key Laboratory of Marine Bioresource and Eco-environmental Science, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
College of Life Sciences and Technology, Hubei Engineering University, Xiaogan, China.
PLoS One. 2019 Nov 7;14(11):e0224926. doi: 10.1371/journal.pone.0224926. eCollection 2019.
Euglena, a new superfood on the market, is a nutrient-rich, green single-celled microorganism that features the characteristics of both plant and animal. When cultivated under different conditions, Euglena produces different bioactive nutrients. Interestingly, Euglena is the only known microorganism whose chloroplasts are easy to lose under stress and become permanently bleached. We applied gas chromatography-mass spectrometry (GC-MS) to determine the metabolomes of wild-type (WT) Euglena gracilis and its bleached mutant OflB2 under light stimulation. We found a significant metabolic difference between WT and OflB2 cells in response to light. An increase of membrane components (phospholipids and acylamides) was observed in WT, while a decrease of some stimulant metabolites was detected in OflB2. These metabolomic changes after light stimulation are of great significance to the development of Euglena chloroplasts and their communications with the nucleus.
市场上的一种新的超级食物——眼虫，是一种营养丰富的绿色单细胞微生物，具有植物和动物的双重特征。当在不同条件下培养时，眼虫会产生不同的生物活性营养物质。有趣的是，眼虫是唯一已知的在受到压力时其叶绿体容易丢失并永久褪色的微生物。我们应用气相色谱-质谱联用（GC-MS）来确定野生型（WT）眼虫和其漂白突变体 OflB2 在光照刺激下的代谢组。我们发现 WT 和 OflB2 细胞对光的反应存在显著的代谢差异。WT 中观察到膜成分（磷脂和酰胺）增加，而 OflB2 中一些刺激代谢物减少。这些光照刺激后的代谢组学变化对于眼虫叶绿体的发育及其与细胞核的通讯具有重要意义。
