Kojima S, Tadenuma H, Inada Y, Saito Y
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
J Cell Physiol. 1989 Jan;138(1):192-6. doi: 10.1002/jcp.1041380125.
A hitherto unknown function of granulocyte colony-stimulating factor (G-CSF) was found using cultured endothelial cells. G-CSF stimulated activity of plasminogen activator (PA) in both extracellular and intracellular milieus of endothelial cells obtained from bovine carotid and aortic artery. This effect was dependent on the concentration of G-CSF added to the culture medium and on the treatment time. The extracellular activity was enhanced approximately 5-fold at a concentration of 5,000 colony-forming unit (CFU)/ml (2.6 nM) and in about a 15-hr treatment period. Analyses by fibrin and reverse fibrin autography revealed that activity of PA was much more increased than that of PA inhibitor in endothelial cells treated with G-CSF.
利用培养的内皮细胞发现了粒细胞集落刺激因子（G-CSF）一种迄今未知的功能。G-CSF刺激了从牛颈动脉和主动脉获取的内皮细胞的细胞外和细胞内环境中的纤溶酶原激活物（PA）的活性。这种作用取决于添加到培养基中的G-CSF的浓度以及处理时间。在浓度为5000集落形成单位（CFU）/毫升（2.6纳摩尔）且处理约15小时的时间段内，细胞外活性增强了约5倍。通过纤维蛋白和反向纤维蛋白自显影分析表明，在用G-CSF处理的内皮细胞中，PA的活性比PA抑制剂的活性增加得更多。
