Rowlands D C
Department of Histopathology, East Birmingham Hospital, Bordesley Green East.
J Clin Pathol. 1988 Nov;41(11):1200-2. doi: 10.1136/jcp.41.11.1200.
The variations in the numbers of nucleolar organising regions (NORs) among different grades of cervical intraepithelial neoplasia (CIN) were investigated using a silver staining technique. Twenty four biopsy specimens were studied (six normal and six of each of the three grades of CIN) by staining paraffin wax sections using a silver (AgNOR) method that stains the NORS as multiple black dots within nuclei (AgNORs). The number of AgNORs in the nuclei of cells in the basal half of the squamous epithelium was counted, and the average number of AgNORs in each cell calculated for each specimen (the AgNOR count). There was no difference in the number of AgNORs in the squamous epithelium of normal biopsy specimens and those showing CIN1 and CIN2, but there was a small but significant increase in the CIN3 group.
采用银染技术研究了不同级别宫颈上皮内瘤变（CIN）中核仁组织区（NORs）数量的变化。通过银（AgNOR）染色法对石蜡切片进行染色，该方法将NORs染成细胞核内的多个黑点（AgNORs），对24份活检标本（6份正常标本以及CIN三个级别各6份）进行了研究。对鳞状上皮基底层细胞细胞核中的AgNORs数量进行计数，并计算每个标本中每个细胞的AgNORs平均数（AgNOR计数）。正常活检标本的鳞状上皮与显示CIN1和CIN2的标本中AgNORs数量没有差异，但CIN3组有小幅但显著的增加。
