Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Amino Acids. 2014 Jun;46(6):1583-7. doi: 10.1007/s00726-014-1713-3. Epub 2014 Mar 15.
L-Theanine is a unique amino acid present in green tea. It elicits umami taste and has a considerable effect on tea taste and quality. We investigated L-theanine activity on the T1R1 + T1R3 umami taste receptor. L-Theanine activated T1R1 + T1R3-expressing cells and showed a synergistic response with inosine 5'-monophosphate. The site-directed mutagenesis analysis revealed that L-theanine binds to L-amino acid binding site in the Venus flytrap domain of T1R1. This study shows that L-theanine elicits an umami taste via T1R1 + T1R3.
L-茶氨酸是绿茶中特有的一种氨基酸。它能产生鲜味，并对茶的味道和品质有相当大的影响。我们研究了 L-茶氨酸对 T1R1+T1R3 鲜味受体的活性。L-茶氨酸激活表达 T1R1+T1R3 的细胞，并与肌苷 5'-单磷酸产生协同反应。定点突变分析表明，L-茶氨酸与 T1R1 的 Venus 飞蝇陷阱结构域中的 L-氨基酸结合位点结合。这项研究表明，L-茶氨酸通过 T1R1+T1R3 产生鲜味。
