Duarte Moreira Raquel Regina, Zimmermann Martins Gilmarcio, Teixeira Botelho Vinícius, dos Santos Luis Eduardo, Cavaleiro Carlos, Salgueiro Lígia, Andrade Géssica, Gomes Martins Carlos Henrique
Departamento de Princípios Ativos Naturais e Toxicologia, Faculdade de Ciências Farmacêuticas - UNESP - Universidade Estadual Paulista, Rodovia Araraquara, Jaú Km 1 s/n Cep: 14901-502, Campus Ville, Araraquara, São Paulo, Brasil, (phone: +55-16-33016992; fax: +55-16-33016980).
Chem Biodivers. 2014 Mar;11(3):438-44. doi: 10.1002/cbdv.201300322.
The chemical composition of the essential oil isolated from the aerial parts of Melampodium divaricatum (Rich.) DC. (Asteraceae) was characterized by GC-FID and GC/MS analyses. (E)-Caryophyllene (56.0%), germacrene D (12.7%), and bicyclogermacrene (9.2%) were identified as the major oil components. The antimicrobial activity of the oil against seven standard strains of oral pathogens from the American Type Culture Collection (ATCC) was evaluated by determining minimum inhibitory concentrations (MICs) using the microdilution method. MIC Values below 100 μg/ml were obtained against Streptococcus sobrinus (90 μg/ml), Lactobacillus casei (30 μg/ml), S. mutans (20 μg/ml), and S. mitis (18 μg/ml). In contrast, the MIC values of the major oil compound (E)-caryophyllene were higher than 400 μg/ml against all pathogens, suggesting that the activity of the oil might depend on minor oil components and/or on synergistic effects. The M. divaricatum essential oil is a promising agent to include in anticariogenic oral rinse formulations for the control of oral pathogens.
对从伞房匹菊（Melampodium divaricatum (Rich.) DC.，菊科）地上部分分离得到的精油进行了化学成分分析，采用气相色谱-火焰离子化检测（GC-FID）和气相色谱/质谱联用（GC/MS）分析方法对其进行了表征。鉴定出（E）-石竹烯（56.0%）、吉马烯D（12.7%）和双环吉马烯（9.2%）为该精油的主要成分。采用微量稀释法测定最低抑菌浓度（MIC），对该精油针对美国典型培养物保藏中心（ATCC）的7种口腔病原菌标准菌株的抗菌活性进行了评估。对远缘链球菌（90μg/ml）、干酪乳杆菌（30μg/ml）、变形链球菌（20μg/ml）和缓症链球菌（18μg/ml）的MIC值均低于100μg/ml。相比之下，主要精油成分（E）-石竹烯对所有病原菌的MIC值均高于400μg/ml，这表明该精油的活性可能取决于次要的精油成分和/或协同效应。伞房匹菊精油有望用于抗龋性口腔含漱制剂中，以控制口腔病原菌。
