EphB4、EphrinB2和表皮生长因子受体在甲状腺乳头状癌中的过表达：一项初步研究。
Overexpression of EphB4, EphrinB2, and epidermal growth factor receptor in papillary thyroid carcinoma: A pilot study.
作者信息
Sharma Giriraj K, Dhillon Vaninder K, Masood Rizwan, Maceri Dennis R
机构信息
Department of Otolaryngology - Head and Neck Surgery, Keck School of Medicine of University of Southern California, Los Angeles, California.
出版信息
Head Neck. 2015 Jul;37(7):964-9. doi: 10.1002/hed.23694. Epub 2014 Jun 27.
BACKGROUND
The purpose of this study was to examine the differential expression of EphB4, EphrinB2, and epidermal growth factor receptor (EGFR) genes in papillary thyroid carcinoma (PTC) and evaluate their association with lymph node metastasis.
METHODS
EphB4, EphrinB2, and EGFR expression in 21 matched tumors and surrounding normal thyroid tissues were evaluated by complementary DNA (cDNA) microarray, Western blot, and immunohistochemistry (IHC).
RESULTS
We noted a statistically significant overexpression of EphB4, EphrinB2, and EGFR in tumor versus normal tissue based on cDNA microarray, Western blot, and IHC analysis. EphB4 and EphrinB2 overexpression were significantly associated with the presence of lymph node disease.
CONCLUSION
Overexpression of EphB4, EphrinB2, and EGFR are associated with PTC, whereas EphB4 and EphrinB2 overexpression are associated with lymph node metastases. These genes may be potential biomarkers for identification of subclinical lymph node involvement in PTC and potential small-molecule targets for pharmacotherapy research.
背景
本研究旨在检测EphB4、EphrinB2和表皮生长因子受体（EGFR）基因在甲状腺乳头状癌（PTC）中的差异表达，并评估它们与淋巴结转移的相关性。
方法
通过互补DNA（cDNA）微阵列、蛋白质免疫印迹法和免疫组织化学（IHC）评估21对匹配的肿瘤组织和周围正常甲状腺组织中EphB4、EphrinB2和EGFR的表达。
结果
基于cDNA微阵列、蛋白质免疫印迹法和免疫组织化学分析，我们发现肿瘤组织中EphB4、EphrinB2和EGFR的表达在统计学上显著高于正常组织。EphB4和EphrinB2的过表达与淋巴结疾病的存在显著相关。
结论
EphB4、EphrinB2和EGFR的过表达与PTC相关，而EphB4和EphrinB2的过表达与淋巴结转移相关。这些基因可能是识别PTC亚临床淋巴结受累的潜在生物标志物，也是药物治疗研究的潜在小分子靶点。
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