Keles Zeynep Pinar, Keles Gonca Cayir, Avci Bahattin, Cetinkaya Burcu Ozkan, Emingil Gulnur
Department of Periodontology, Faculty of Dentistry, Ondokuzmayis University, Samsun, Turkey.
J Periodontol. 2014 Sep;85(9):1240-6. doi: 10.1902/jop.2014.130631. Epub 2014 Mar 17.
YKL-40, a new acute-phase protein, is shown to be elevated in inflammatory diseases, such as rheumatoid arthritis, type 2 diabetes mellitus, and coronary artery diseases. However, there is no data indicating a relationship between YKL-40 and periodontal disease. Interleukin-6 (IL-6) is the major regulator of acute-phase protein synthesis and one of the most studied inflammatory markers in periodontal disease. The purpose of the present study is to evaluate YKL-40 and IL-6 levels in gingival crevicular fluid (GCF) and serum of patients with periodontal disease and healthy individuals.
Periodontally healthy individuals (n = 15), patients with gingivitis (n = 15), and patients with severe chronic periodontitis (CP) (n = 15) without any systemic disease were included in the study. Clinical measurements were recorded; GCF and blood samples were obtained from each participant. GCF and serum YKL-40 and IL-6 levels were analyzed by enzyme-linked immunosorbent assay. Statistical analysis was performed by parametric and non-parametric tests.
Total amounts of YKL-40 and IL-6 in GCF as well as serum YKL-40 and IL-6 levels were significantly higher in patients with gingivitis and CP compared with healthy controls (P <0.01). YKL-40 levels in GCF and serum as well as serum IL-6 levels were significantly higher in patients with CP compared with patients with gingivitis (P <0.01).
YKL-40 levels in GCF as well as serum YKL-40 and IL-6 levels increased from gingivitis to periodontitis. Within the limits of the present study, the YKL-40 molecule might be a potential novel inflammatory marker of periodontal disease.
YKL-40是一种新的急性期蛋白，在类风湿关节炎、2型糖尿病和冠状动脉疾病等炎症性疾病中水平升高。然而，尚无数据表明YKL-40与牙周病之间存在关联。白细胞介素-6（IL-6）是急性期蛋白合成的主要调节因子，也是牙周病中研究最多的炎症标志物之一。本研究的目的是评估牙周病患者和健康个体龈沟液（GCF）及血清中YKL-40和IL-6的水平。
本研究纳入了15名牙周健康个体、15名牙龈炎患者和15名无任何全身性疾病的重度慢性牙周炎（CP）患者。记录临床测量数据；从每位参与者采集GCF和血液样本。采用酶联免疫吸附测定法分析GCF和血清中YKL-40和IL-6的水平。通过参数检验和非参数检验进行统计分析。
与健康对照组相比，牙龈炎患者和CP患者的GCF中YKL-40和IL-6总量以及血清YKL-40和IL-6水平显著更高（P<0.01）。与牙龈炎患者相比，CP患者的GCF和血清中YKL-40水平以及血清IL-6水平显著更高（P<0.01）。
从牙龈炎到牙周炎，GCF中YKL-40水平以及血清YKL-40和IL-6水平均升高。在本研究范围内，YKL-40分子可能是牙周病一种潜在的新型炎症标志物。
