College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China.
College of Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, China; College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, China.
Vet Parasitol. 2014 May 28;202(3-4):241-7. doi: 10.1016/j.vetpar.2014.01.028. Epub 2014 Feb 7.
A marasmic pig with watery diarrhea was identified to harbor the human-pathogenic protist Pentatrichomonas hominis by PCR and sequence analysis of three genetic loci (ITS1-5.8S rRNA-ITS2, 18S rRNA, and EF-1α), electron microscopy, and infection experiments. DNA sequencing and phylogenetic analysis indicated the organism isolated in this study was most closely related to P. hominis. SEM and TEM observation of the ultrastructure demonstrated that it had a morphology identical to P. hominis. The result of experimental infections with P. hominis exhibited that the cells had the ability to propagate in the cecum of piglets and fecal-oral route might be the major way in which pigs became infected. The present study confirmed that swine could be a host for P. hominis and might serve as a reservoir for human trichomoniasis.
通过 PCR 和对三个遗传基因座（ITS1-5.8S rRNA-ITS2、18S rRNA 和 EF-1α）、电子显微镜和感染实验的序列分析，鉴定出一头患有消瘦性水样腹泻的病猪携带了人病原原生动物五毛滴虫。DNA 测序和系统发育分析表明，本研究中分离到的病原体与五毛滴虫最为密切相关。超微结构的 SEM 和 TEM 观察表明，它的形态与五毛滴虫完全相同。用五毛滴虫进行的实验感染结果表明，这些细胞能够在仔猪的盲肠中繁殖，粪-口途径可能是猪感染的主要途径。本研究证实猪可以作为五毛滴虫的宿主，可能是人类滴虫病的储存宿主。
