Department of Biotechnology, Chemistry, and Environmental Engineering, Aalborg University, Sohngaardholmsvej 49, DK-9000 Aalborg, Denmark.
Department of Otorhinolaryngology, Head and Neck Surgery and Audiology, Rigshospitalet, University of Copenhagen, Blegdamsvej 9, DK-2100 Copenhagen, Denmark.
J Cyst Fibros. 2014 Dec;13(6):645-52. doi: 10.1016/j.jcf.2014.02.008. Epub 2014 Mar 11.
For the first time microorganisms in CF sinuses are investigated by molecular methods in response to an absence of anaerobes in CF sinus samples during a two-year period at the Copenhagen CF center.
Endoscopic sinus surgery was performed in 19 CF patients. DNA from intact bacterial cells was investigated by 16S rRNA gene analysis and quantitative PCR. Results were compared to culture-dependent routine diagnosis.
Molecular methods showed a large microbial diversity, which included undetected anaerobes that may play a pathogenic role. Importantly, the culture methods did not always detect known CF pathogens. Quantitative PCR showed generally a higher abundance of classic CF pathogens e.g. Pseudomonas aeruginosa and Staphylococcus aureus compared with the anaerobe Propionibacterium acnes.
The results indicate that the culture methods in some cases may not be suitable as stand-alone method for this patient group, as diversity may be underestimated and important species undetected.
为首次通过分子方法研究 CF 鼻窦中的微生物，我们对哥本哈根 CF 中心在两年期间 CF 鼻窦样本中缺乏厌氧菌的情况进行了调查。
对 19 例 CF 患者进行了内窥镜鼻窦手术。通过 16S rRNA 基因分析和定量 PCR 研究完整细菌细胞的 DNA。将结果与基于培养的常规诊断进行了比较。
分子方法显示出微生物的多样性，其中包括可能具有致病作用的未检出厌氧菌。重要的是，培养方法并非总能检测到已知的 CF 病原体。定量 PCR 显示，与普通的 CF 病原体（如铜绿假单胞菌和金黄色葡萄球菌）相比，经典 CF 病原体如丙酸杆菌的丰度通常更高。
结果表明，在某些情况下，培养方法可能不适合作为该患者群体的独立方法，因为多样性可能被低估，重要的物种可能未被检出。
