Department of Microbiology & Immunology, University of Minnesota, USA.
Department of Otolaryngology, Head and Neck Surgery, University of Minnesota, USA.
J Cyst Fibros. 2018 Mar;17(2):204-212. doi: 10.1016/j.jcf.2017.08.007. Epub 2017 Aug 18.
Metastasis of upper airway microbiota may have significant implications in the development of chronic lung disease. Here, we compare bacterial communities of matched sinus and lung mucus samples from cystic fibrosis (CF) subjects undergoing endoscopic surgery for treatment of chronic sinusitis.
Mucus from one maxillary sinus and expectorated sputum were collected from twelve patients. 16S rRNA gene sequencing was then performed on sample pairs to compare the structure and function of CF airway microbiota.
Bacterial diversity was comparable between airway sites, though sinuses harbored a higher prevalence of dominant microorganisms. Ordination analyses revealed that samples clustered more consistently by airway niche rather than by individual. Finally, predicted metagenomes suggested that anaerobiosis was enriched in the lung.
Our findings indicate that while the lung may be seeded by individual sinus pathogens, airway microenvironments harbor distinct bacterial communities that should be considered in selecting antimicrobial therapies.
上呼吸道微生物群的转移可能对慢性肺部疾病的发展有重要影响。在这里，我们比较了接受内镜手术治疗慢性鼻窦炎的囊性纤维化（CF）患者配对的鼻窦和肺粘液样本中的细菌群落。
从 12 名患者中收集了一个上颌窦和咳出的痰的粘液。然后对样本对进行 16S rRNA 基因测序，以比较 CF 气道微生物群的结构和功能。
气道部位的细菌多样性相当，但鼻窦中存在更高比例的优势微生物。排序分析表明，样本的聚类更一致地通过气道生态位而不是个体。最后，预测的宏基因组表明，肺部的厌氧性更为丰富。
我们的研究结果表明，尽管肺部可能被个别鼻窦病原体定植，但气道微环境中存在独特的细菌群落，在选择抗菌治疗时应加以考虑。
