Shanahan Michael T, Carroll Ian M, Gulati Ajay S
Department of Medicine; Division of Gastroenterology and Hepatology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
Department of Pediatrics; Division of Gastroenterology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
Gut Microbes. 2014 Mar-Apr;5(2):208-14. doi: 10.4161/gmic.27466. Epub 2013 Dec 20.
Paneth cells are long-lived secretory cells that reside in the base of the crypts of Lieberkühn of the small intestine. They produce an arsenal of molecules that are involved in numerous biological processes, ranging from the control of gut microbial populations to supporting the intestinal stem cell niche. Because of these important functions, Paneth cell abnormalities are becoming implicated in a variety of disease processes. As such, it is necessary to establish parameters that will allow for the comprehensive study of Paneth cells in health and disease. In this addendum, we highlight critical design aspects involved in the study of Paneth cells and their downstream effects on the intestinal microbiota. The importance of this approach is demonstrated by our recent findings that Nod2 does not regulate mouse Paneth cell antimicrobial function, in contrast to previous reports. This work defines key issues to consider when studying Paneth cells in mouse systems.
潘氏细胞是一种长寿的分泌细胞，位于小肠利伯库恩隐窝的底部。它们产生一系列分子，这些分子参与众多生物过程，从控制肠道微生物群落到支持肠道干细胞生态位。由于这些重要功能，潘氏细胞异常正被认为与多种疾病过程有关。因此，有必要建立一些参数，以便对健康和疾病状态下的潘氏细胞进行全面研究。在本附录中，我们强调了潘氏细胞研究及其对肠道微生物群下游影响所涉及的关键设计方面。我们最近的研究结果表明，与之前的报道相反，Nod2并不调节小鼠潘氏细胞的抗菌功能，这一方法的重要性由此得到了证明。这项工作定义了在小鼠系统中研究潘氏细胞时需要考虑的关键问题。
