Kaur Gurbinder, Pickrell G, Kimsawatde G, Homa D, Allbee H A, Sriranganathan N
Department of Material Science and Engineering, Holden Hall, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
Department of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia-24060, USA.
Sci Rep. 2014 Mar 18;4:4392. doi: 10.1038/srep04392.
CaO-P2O5-SiO2-B2O3-ZnO bioactive glasses were prepared via an optimized sol-gel method. The current investigation was focused on producing novel zinc based calcium phosphoborosilicate glasses and to evaluate their mechanical, rheological, and biocompatible properties. The morphology and composition of these glasses were studied using X-ray diffraction (XRD) and scanning electron microscopy (SEM). The particle size, mechanical and flexural strength was also determined. Furthermore, the zeta potential of all the glasses were determined to estimate their flocculation tendency. The thermal analysis and weight loss measurements were carried out using differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) respectively. For assessing the in-vitro bioactive character of synthesized glasses, the ability for apatite formation on their surface upon their immersion in simulated body fluid (SBF) was checked using SEM and pH measurements. MTS assay cytotoxicity assay and live-dead cell viability test were conducted on J774A.1 cells murine macrophage cells for different glass concentrations.
通过优化的溶胶-凝胶法制备了CaO-P2O5-SiO2-B2O3-ZnO生物活性玻璃。当前的研究重点是生产新型锌基钙磷硼硅酸盐玻璃，并评估其机械、流变学和生物相容性。使用X射线衍射（XRD）和扫描电子显微镜（SEM）研究了这些玻璃的形态和组成。还测定了粒径、机械强度和抗弯强度。此外，测定了所有玻璃的zeta电位以估计其絮凝倾向。分别使用差示扫描量热法（DSC）和热重分析（TGA）进行热分析和失重测量。为了评估合成玻璃的体外生物活性特征，使用SEM和pH测量检查了它们浸入模拟体液（SBF）后在其表面形成磷灰石的能力。针对不同玻璃浓度，在J774A.1细胞（小鼠巨噬细胞）上进行了MTS细胞毒性测定和活死细胞活力测试。
