Lee Daeun, Kim Mi-Sun, Park Jimin, Jhon Gil-Ja, Son Jin H, Shin Dong Hae
College of Pharmacy and Graduate School of Pharmaceutical Sciences, Global Top5 Research Program, Ewha Womans University, Seoul 120-750, Korea.
Department of Chemistry and Nano Science, Global Top5 Research Program, Ewha Womans University, Seoul 120-750, Korea.
Int J Mol Sci. 2014 Mar 14;15(3):4523-30. doi: 10.3390/ijms15034523.
Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 Å using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P21212, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.
肿瘤坏死因子α诱导蛋白8/氧化应激调节基因-α（Oxi-α）是一种在脑多巴胺能神经元中特异性表达的新型蛋白质，据报道其过表达可保护多巴胺能细胞免受氧化应激诱导的细胞死亡。在本研究中，小鼠C165S突变型Tnfaip8/Oxi-α已结晶，并利用同步辐射收集到了分辨率为1.8 Å的X射线数据。该晶体属于原始正交空间群P21212，晶胞参数为a = 66.9、b = 72.3、c = 93.5 Å。目前正在进行完整的结构测定，以便深入了解该蛋白质的结构-功能关系。
