Huang Jia, Ye Xiaoqian, You Yongmei, Liu Weilin, Gao Yanling, Yang Shanli, Peng Jun, Hong Zhenfeng, Tao Jing, Chen Lidian
College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
MOE Key Laboratory of Traditional Chinese Medicine on Osteology and Traumatology and Exercise Rehabilitation, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, P.R. China.
Int J Mol Med. 2014 Jun;33(6):1547-53. doi: 10.3892/ijmm.2014.1702. Epub 2014 Mar 18.
The aim of this study was to investigate the effect of electroacupuncture (EA) on cell proliferation and its molecular mechanisms. Sixty rats were randomly divided into 5 groups: sham operation control (SC), ischemia control (IC), EA, EA and DMSO injection (ED), EA and U0126 injection (EU). All the groups, with the exception of SC, underwent middle cerebral artery occlusion (MCAO), and DMSO or U0126 was injected into the rat in the ED or EU group 30 min prior to MCAO. Cell proliferation was evaluated by proliferating cell nuclear antigen (PCNA) immunostaining. The changes of cell cycle proteins (cyclin D1, CDK4, cyclin E, CDK2, p21 and p27) and the ERK1/2 pathway activation were examined by RT-PCR and western blot analysis. The results showed that the positive cell numbers of PCNA immunostaining in the EA and ED groups were more than those in the IC group (P<0.05). The mRNA and protein levels of p21 or p27 were obviously increased, however, the mRNA and protein levels of cyclin D1, CDK4, cyclin E and CDK2 were reduced in the IC and EU groups. The findings suggested that EA activates the ERK1/2 signaling pathway to protect brain injury during cerebral ischemia. However, this positive effect of EA can be blocked by U0126.
本研究旨在探讨电针（EA）对细胞增殖的影响及其分子机制。将60只大鼠随机分为5组：假手术对照组（SC）、缺血对照组（IC）、电针组（EA）、电针加二甲基亚砜注射组（ED）、电针加U0126注射组（EU）。除SC组外，其余各组均行大脑中动脉闭塞（MCAO）手术，ED组和EU组在MCAO术前30分钟向大鼠注射二甲基亚砜或U0126。通过增殖细胞核抗原（PCNA）免疫染色评估细胞增殖情况。采用逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析检测细胞周期蛋白（细胞周期蛋白D1、细胞周期蛋白依赖性激酶4、细胞周期蛋白E、细胞周期蛋白依赖性激酶2、p21和p27）的变化以及细胞外信号调节激酶1/2（ERK1/2）信号通路的激活情况。结果显示，EA组和ED组PCNA免疫染色阳性细胞数多于IC组（P<0.05）。IC组和EU组中，p21或p27的mRNA和蛋白水平明显升高，而细胞周期蛋白D1、细胞周期蛋白依赖性激酶4、细胞周期蛋白E和细胞周期蛋白依赖性激酶2的mRNA和蛋白水平降低。研究结果表明，电针激活ERK1/2信号通路以保护脑缺血期间的脑损伤。然而，电针的这种积极作用可被U0126阻断。
