Nabi Ali, Khalili Mohammad Ali, Halvaei Iman, Ghasemzadeh Jalal, Zare Ehsan
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2013 Nov;11(11):925-32.
It is estimated that about 50% of causes of recurrent pregnancy loss (RPL) cases remain unknown. Sperm factors are suggested to have probable role in cases with RPL.
The goal was to determine the possible relationship between semen bacterial contaminations with unexplained RPL. Also, the correlation between number of bacterial colony and sperm chromatin condensation was examined.
This study consisted of 30 fertile men (group A) and 30 infertile (group B) men with unknown RPL. Semen collection and analysis were done according to WHO manuals. Sperm count and motility were evaluated by Makler chamber. Eosin-Nigrosin and Papanicolaou staining methods were applied for viability and morphology assessment, respectively. The semen samples from both groups were cultured for aerobic bacteria. Aniline blue (AB) and toluidine blue (TB) staining methods were applied for evaluating sperm chromatin condensation.
The numbers of colonies were significantly higher in group B when compared to group A. Also, S. aureus and E. coli contaminations showed significant differences between two groups. Both AB+ and TB+ sperm cells showed significant increase in group B compared to group A. There was a significant negative correlation between colony number and progressive motility (p=0.01), and sperm viability (p=0.007). In addition, positive correlations were found between colony number and AB(+) (p=0.001) and TB+ (p=0.004) as well.
Bacterial contaminations in semen of men from RPL couples had significantly higher levels when compared to fertile controls. Presence of microorganisms in semen may be correlated with irregular sperm parameters and quality.
据估计，约50%的复发性流产（RPL）病例病因不明。精子因素被认为在RPL病例中可能起作用。
确定精液细菌污染与不明原因RPL之间的可能关系。此外，还研究了细菌菌落数与精子染色质凝聚之间的相关性。
本研究包括30名有生育能力的男性（A组）和30名不明原因RPL的不育男性（B组）。精液采集和分析按照世界卫生组织手册进行。精子计数和活力通过Makler计数板评估。分别采用伊红 - 黑色素和巴氏染色法评估精子活力和形态。两组的精液样本均进行需氧菌培养。采用苯胺蓝（AB）和甲苯胺蓝（TB）染色法评估精子染色质凝聚。
与A组相比，B组的菌落数显著更高。此外，金黄色葡萄球菌和大肠杆菌污染在两组之间存在显著差异。与A组相比，B组中AB +和TB +精子细胞均显著增加。菌落数与进行性活力（p = 0.01）和精子活力（p = 0.007）之间存在显著负相关。此外，菌落数与AB(+)（p = 0.001）和TB +（p = 0.004）之间也存在正相关。
与有生育能力的对照组相比，RPL夫妇男性精液中的细菌污染水平显著更高。精液中微生物的存在可能与精子参数和质量异常有关。
