Mirzaei Jafar, Movahedin Mansoureh, Halvaei Iman
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Email:
Cell J. 2022 Jun;24(6):330-336. doi: 10.22074/cellj.2022.8119. Epub 2022 Jun 29.
Sperm cryopreservation results in damage to membrane integrity, sperm viability, sperm motility, and DNA structure. We aimed to evaluate the effect of plasma rich in growth factors (PRGF) on sperm parameters during the freeze-thaw process.
In the first phase of this prospective study, after sperm preparation, 10 normozoospermic specimens were cryopreserved by rapid freezing with different concentrations of PRGF including 0, 1, 5, and 10% to find the optimum dose. Sperm motility and viability were assessed in this phase. In the second phase of the study, based on the results of first phase, 25 normal sperm samples were frozen with 1% PRGF. All sperm parameters including motility, viability, acrosome reaction, and DNA integrity were assessed before freezing and after thawing.
The rates of progressive and total sperm motility and viability were significantly higher in 1% PRGF compared to control, 5%, and 10% PRGF in the first phase (P<0.05). Supplementation of freezing medium with 1% PRGF could significantly improve all sperm parameters including sperm motility, viability, normal morphology, acrosome integrity, chromatin structure, chromatin integrity, DNA denaturation, and DNA fragmentation in comparison with the control group.
It appears that the supplementation of freezing medium with 1% PRGF could protect human sperm parameters during cryopreservation.
精子冷冻保存会导致膜完整性、精子活力、精子运动能力和DNA结构受损。我们旨在评估富含生长因子的血浆（PRGF）在冻融过程中对精子参数的影响。
在这项前瞻性研究的第一阶段，精子制备后，将10份正常精子标本用不同浓度（0%、1%、5%和10%）的PRGF快速冷冻保存，以确定最佳剂量。此阶段评估精子运动能力和活力。在研究的第二阶段，根据第一阶段的结果，将25份正常精子样本用1%的PRGF冷冻保存。在冷冻前和解冻后评估所有精子参数，包括运动能力、活力、顶体反应和DNA完整性。
在第一阶段，1% PRGF组的精子进行性运动率、总运动率和活力显著高于对照组、5% PRGF组和10% PRGF组（P<0.05）。与对照组相比，在冷冻培养基中添加1% PRGF可显著改善所有精子参数，包括精子运动能力、活力、正常形态、顶体完整性、染色质结构、染色质完整性、DNA变性和DNA片段化。
在冷冻培养基中添加1% PRGF似乎可以在冷冻保存过程中保护人类精子参数。
