Alizadeh Zohreh, Faramarzi Shamila, Saidijam Massoud, Alizamir Tahereh, Esna-Ashari Farzaneh, Shabab Nooshin, Farimani Sanoee Marzieh
Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Obstetrics and Gynecology, Hamadan University of Medical Sciences, Hamadan, Iran.
Iran J Reprod Med. 2013 Dec;11(12):983-8.
HOXA11 and HOXA10 are expressed in endometrium throughout the menstrual cycle and show a dramatic increase during the mid-luteal phase at the time of implantation. The expression of these genes is decreased in women with myomas.
To determine whether myomectomy would reverse HOXA11 and HOXA10 expression, we evaluated the transcript levels of these genes in the endometria of patients before and after myomectomy.
Expression of HOXA11 and HOXA10 were examined prospectively during the midluteal phase in endometrium obtained from infertile women (n=12) with myoma before and three months after myomectomy. Endometrial HOXA11 and HOXA10 expression were evaluated using quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR).
Endometrial HOXA11 and HOXA10 mRNAs expression levels (normalized to 18SrRNA) were increased insignificantly in endometrium of patients after myomectomy (p=0.7 and p=0.15 respectively).
The results suggest that the alteration in expression pattern of these genes could not account for some aspects of fertility after myomectomy. This article extracted from M.Sc. thesis. (Shamila Faramarzi).
HOXA11和HOXA10在整个月经周期的子宫内膜中均有表达，且在植入期的黄体中期显著增加。这些基因在患有子宫肌瘤的女性中表达降低。
为了确定子宫肌瘤切除术是否会逆转HOXA11和HOXA10的表达，我们评估了子宫肌瘤切除术前和术后患者子宫内膜中这些基因的转录水平。
前瞻性地检测了12例患有子宫肌瘤的不孕女性在黄体中期子宫肌瘤切除术前和术后三个月子宫内膜中HOXA11和HOXA10的表达。使用定量实时逆转录聚合酶链反应（RT-PCR）评估子宫内膜中HOXA11和HOXA10的表达。
子宫肌瘤切除术后患者子宫内膜中HOXA11和HOXA10 mRNA的表达水平（以18SrRNA标准化）无显著增加（分别为p = 0.7和p = 0.15）。
结果表明，这些基因表达模式的改变不能解释子宫肌瘤切除术后生育能力的某些方面。本文摘自理学硕士论文。（沙米拉·法拉马尔齐）
