Wang Meimei, Hao Cuifang, Huang Xin, Bao Hongchu, Qu Qinglan, Liu Zhenteng, Dai Huangguan, He Shunzhi, Yan Wei
1 Shandong University School of Medicine, Jinan, People's Republic of China.
2 Reproductive Medicine Centre, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, People's Republic of China.
Reprod Sci. 2018 May;25(5):654-661. doi: 10.1177/1933719117734320. Epub 2017 Oct 11.
This study aimed to study the expression of homeobox (HOX)A11-AS1 ( HOXA11 antisense RNA) long noncoding RNA (lncRNA) and the expression of homeobox A ( HOXA9, HOXA10, HOXA11, and HOXA13) genes in the eutopic (EU) and ectopic (EC) endometria of women with peritoneal endometriosis. A total of 30 women undergoing laparoscopic surgery for peritoneal endometriosis and 15 infertile women without endometriosis were enrolled in this study. Peritoneal EC tissue samples were obtained through surgery. The EU tissues were obtained by curettage. The EC and EU lncRNA and messenger RNA (mRNA) expression levels were measured using real-time reverse transcriptase-polymerase chain reaction. The HOXA11-AS1 lncRNA and HOXA9, HOXA10, HOXA11, and HOXA13 mRNA were expressed at significantly lower levels in the EU than in the EC, that is, in women with peritoneal endometriosis ( P < .05). The expression levels of HOXA10 and HOXA11 in the EU were significantly lower in women with peritoneal endometriosis compared to the control group participants ( P < .05), whereas the levels of lncRNA ( HOXA11-AS1), HOXA9, and HOXA13 did not differ significantly between the 2 patient groups ( P > .05). In conclusion, the study findings suggest that HOXA11-AS1 lncRNA may play a role in the development of peritoneal endometriosis, but HOXA11-AS1 may not influence endometrial receptivity in endometriosis-associated infertility.
本研究旨在探讨同源框（HOX）A11反义RNA（HOXA11-AS1）长链非编码RNA（lncRNA）以及同源框A（HOXA9、HOXA10、HOXA11和HOXA13）基因在腹膜型子宫内膜异位症患者在位（EU）和异位（EC）子宫内膜中的表达情况。本研究共纳入30例行腹腔镜手术治疗腹膜型子宫内膜异位症的女性患者以及15例无子宫内膜异位症的不孕女性。通过手术获取腹膜EC组织样本，通过刮宫获取EU组织。采用实时逆转录聚合酶链反应检测EC和EU组织中lncRNA及信使核糖核酸（mRNA）的表达水平。HOXA11-AS1 lncRNA以及HOXA9、HOXA10、HOXA11和HOXA13 mRNA在EU中的表达水平显著低于EC，即低于腹膜型子宫内膜异位症患者（P<0.05）。与对照组参与者相比，腹膜型子宫内膜异位症患者EU中HOXA10和HOXA11的表达水平显著降低（P<0.05），而lncRNA（HOXA11-AS1）、HOXA9和HOXA13在两组患者中的水平差异无统计学意义（P>0.05）。总之，研究结果表明，HOXA11-AS1 lncRNA可能在腹膜型子宫内膜异位症的发生发展中起作用，但HOXA11-AS1可能不影响子宫内膜异位症相关性不孕患者的子宫内膜容受性。
