Badkoobeh Puran, Parivar Kazem, Kalantar Seyed Mehdi, Hosseini Seyed Davood, Salabat Alireza
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Iran J Reprod Med. 2013 May;11(5):355-64.
Doxorubicin (DOX), an anthracycline antibiotic, is a widely used anticancer agent. In spite of its high antitumor efficacy, the use of DOX in clinical chemotherapy is limited due to diverse toxicities, including gonadotoxicity.
We investigated the protective effect of nano-zinc oxide (nZnO) as an established antioxidant on DOX-induced testicular disorders.
In this experimental study 24 adult male Wistar rats were divided into four groups including one control and three experimentals (6 rats per group). They received saline (as control), DOX alone (6 mg/kg body weight, i.p.), nZnO alone (5 mg/kg body weight, i.p.), and nZnO followed by DOX. Animals were sacrificed 28 days after treatment and evaluations were made by sperm count and measuring sex hormone levels in plasma. Also total antioxidant power (TAP) and lipid peroxidation (LPO) in plasma were tested. Data was analyzed with SPSS-14 and one way ANOVA test. P<0.05 were considered to be statistically significant.
In the DOX-exposed rats significant differences were found compared with the control group (p=0.001) in plasma total antioxidant power (TAP) (425.50±32.33 vs. 493.33±18.54 mmol/mL), Lipid peroxidation (LPO) (3.70±0.44 vs. 2.78±0.68 μmol/mL), plasma testosterone (3.38±0.69 vs. 5.40±0.89 ng/dl), LH (0.26±0.05 vs. 0.49±0.18 mlU/mL), sperm count (157.98±6.29 vs. 171.71±4.42×10(6)/mL) and DNA damage (11.51±3.45 vs. 6.04±2.83%). Co-administration of nZnO significantly improved DOX-induced changes (p=0.013) in plasma TAP (471.83±14.51 mmol/mL), LPO (2.83±0.75 μmol/mL), plasma testosterone (5.00±1.07 ng/dl), LH (0.52±0.08 mlU/mL), sperm count (169.13±5.01×10(6)/mL) and DNA damage (7.00±1.67%).
At the dose designed in the present investigation cytoprotective role of nano-zinc oxide through its antioxidant potential is illuminated in DOX-induced male gonadotoxicity.
阿霉素（DOX）是一种蒽环类抗生素，是一种广泛使用的抗癌药物。尽管其具有较高的抗肿瘤疗效，但由于包括性腺毒性在内的多种毒性，DOX在临床化疗中的应用受到限制。
我们研究了作为一种公认的抗氧化剂的纳米氧化锌（nZnO）对DOX诱导的睾丸疾病的保护作用。
在本实验研究中，将24只成年雄性Wistar大鼠分为四组，包括一组对照组和三组实验组（每组6只大鼠）。它们分别接受生理盐水（作为对照）、单独的DOX（6mg/kg体重，腹腔注射）、单独的nZnO（5mg/kg体重，腹腔注射）以及nZnO后再给予DOX。治疗28天后处死动物，并通过精子计数和测量血浆中性激素水平进行评估。同时还检测了血浆中的总抗氧化能力（TAP）和脂质过氧化（LPO）。数据用SPSS - 14软件和单因素方差分析进行分析。P<0.05被认为具有统计学意义。
与对照组相比，DOX处理的大鼠在血浆总抗氧化能力（TAP）（425.50±32.33 vs. 493.33±18.54mmol/mL）、脂质过氧化（LPO）（3.70±0.44 vs. 2.78±0.68μmol/mL）、血浆睾酮（3.38±0.69 vs. 5.40±0.89ng/dl）、促黄体生成素（LH）（0.26±0.05 vs. 0.49±0.18mIU/mL）、精子计数（157.98±6.29 vs. 171.71±4.42×10⁶/mL）和DNA损伤（11.51±3.45 vs. 6.04±2.83%）方面存在显著差异（p = 0.001）。nZnO与DOX联合给药显著改善了DOX诱导的血浆TAP（471.83±14.51mmol/mL）、LPO（2.83±0.75μmol/mL）、血浆睾酮（5.00±1.07ng/dl）、LH（0.52±0.08mIU/mL）、精子计数（169.13±5.01×10⁶/mL）和DNA损伤（7.00±1.67%）的变化（p = 0.013）。
在本研究设计的剂量下，纳米氧化锌通过其抗氧化潜力在DOX诱导的雄性性腺毒性中发挥细胞保护作用。
