Ebadi Manas Ghodrat, Hasanzadeh Shapour, Najafi Golamreza, Parivar Kazem, Yaghmaei Parichehr
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Basic Veterinary Sciences, Histology and Embryology Sections, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Iran J Reprod Med. 2013 Aug;11(8):605-10.
Pyridaben, a pyridazinone derivative, is a new acaricide and insecticide for control of mites and some insects such as white flies, aphids and thrips.
This study was designed to elucidate how pyridaben can affect the sperms' morphological parameters, its DNA integrity, and to estimate the effect of various quantities of pyridaben on in vitro fertilization rate.
In this study, 80 adult male Balb/C strain mice were used. Animals were divided into control and two test groups. Control group received distilled water. The test group was divided into two subgroups, viz, high dose (212 mg/kg/day) and low dose (53 mg/kg/day) and they received the pyridaben, orally for duration of 45 days. The spermatozoa were obtained from caudae epididymides on day 45 in all groups. Sperm viability, protamin compression (nuclear maturity), DNA double-strand breaks, and in vitro fertilizing (IVF) ability were examined.
The pyridaben treatment provoked a significant decrease in sperm population and viability in epididymides. The data obtained from this experiment revealed that, the pyridaben brings about negative impact on the sperm maturation and DNA integrity in a time-dependent manner, which consequently caused a significant (p<0.05) reduction in IVF capability. Embryo developing arrest was significantly (p<0.05) higher in treated than the control group.
Theses results confirmed that, the pyridaben is able to induce DNA damage and chromatin abnormalities in spermatozoa which were evident by low IVF rate. This article extracted from Ph.D. thesis. (Ghodrat Ebadi Mans).
哒螨灵是一种哒嗪酮衍生物，是一种新型杀螨剂和杀虫剂，可用于防治螨虫以及一些昆虫，如粉虱、蚜虫和蓟马。
本研究旨在阐明哒螨灵如何影响精子的形态参数及其DNA完整性，并评估不同剂量的哒螨灵对体外受精率的影响。
本研究使用了80只成年雄性Balb/C品系小鼠。动物被分为对照组和两个试验组。对照组给予蒸馏水。试验组分为两个亚组，即高剂量组（212毫克/千克/天）和低剂量组（53毫克/千克/天），它们口服哒螨灵，持续45天。在第45天从所有组的附睾尾部获取精子。检测精子活力、鱼精蛋白压缩（核成熟度）、DNA双链断裂以及体外受精（IVF）能力。
哒螨灵处理导致附睾中精子数量和活力显著下降。从该实验获得的数据显示，哒螨灵对精子成熟和DNA完整性产生时间依赖性的负面影响，从而导致体外受精能力显著（p<0.05）降低。处理组胚胎发育停滞显著（p<0.05）高于对照组。
这些结果证实，哒螨灵能够诱导精子中的DNA损伤和染色质异常，这在低体外受精率中表现明显。本文摘自博士论文。（戈德拉特·埃巴迪·曼斯）
