Zheng Weiwei, General Surgery Department, The First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
Chen Liping, General Surgery Department, The First Affiliated Hospital of Xinxiang Medical University, Weihui, China.
Pak J Med Sci. 2014 Jan;30(1):106-10. doi: 10.12669/pjms.301.4189.
we conducted a case-control study to investigate the association between dietary folate, vitamin B6 and vitamin B12 intake, MTHFR and MTR genotype, and breast cancer risk.
Genotyping for MTHFR C677T and A1298C and MTR A2756G polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) method. The intake of folate, vitamin B6 and vitamin B12 were calculated by each food item from questionnaire.
Subjects with breast cancer tended to have more first-degree relatives (χ(2) =30.77, P<0.001) and have high intake of folate (t=2.42, P=0.008) and Vitamin B6 (t=2.94, P=0.002). Compared to the reference group, women with MTHFR 677 TT genotype and T allele had a significantly increased risk of breast cancer, with ORs (95%CI) of 1.8(1.08-2.27) and 1.39(1.02-1.92), respectively. For those who had folate intake＜450 ug/day, MTHFR 667TT genotype was associated with a higher risk of breast cancer (OR=2.45, 95% CI=1.09-5.82, P=0.02). Similarly, subjects with Vitamin B6 intake＜0.84 mg/day and MTHFR 667T allele genotype was correlated with a marginally increased risk of breast cancer. A significant interaction was observed between MTHFR C667T polymorphism and folate intake on the risk of breast cancer (P for interaction was 0.025).
This case-control study found a significant association between MTHFR C667T polymorphism, folate intake and vitamin B6 and breast cancer risk, and a significant interaction was observed between MTHFR C667T polymorphism and folate intake on the risk of breast cancer.
我们进行了一项病例对照研究，以调查膳食叶酸、维生素 B6 和维生素 B12 摄入、MTHFR 和 MTR 基因型与乳腺癌风险之间的关系。
使用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）方法对 MTHFR C677T 和 A1298C 以及 MTR A2756G 多态性进行基因分型。通过问卷中的每个食物项目计算叶酸、维生素 B6 和维生素 B12 的摄入量。
患有乳腺癌的受试者往往有更多的一级亲属（χ²=30.77，P<0.001），并且叶酸（t=2.42，P=0.008）和维生素 B6（t=2.94，P=0.002）摄入量较高。与对照组相比，MTHFR 677 TT 基因型和 T 等位基因的女性患乳腺癌的风险显著增加，比值比（95%CI）分别为 1.8（1.08-2.27）和 1.39（1.02-1.92）。对于叶酸摄入量<450ug/天的人，MTHFR 667TT 基因型与乳腺癌风险较高相关（OR=2.45，95%CI=1.09-5.82，P=0.02）。同样，维生素 B6 摄入量<0.84mg/天和 MTHFR 667T 等位基因的受试者与乳腺癌的风险略有增加相关。MTHFR C667T 多态性与叶酸摄入量对乳腺癌风险的交互作用具有统计学意义（交互作用的 P 值为 0.025）。
这项病例对照研究发现，MTHFR C667T 多态性、叶酸摄入和维生素 B6 与乳腺癌风险之间存在显著关联，并且 MTHFR C667T 多态性与叶酸摄入对乳腺癌风险的交互作用具有统计学意义。
