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过表达丙二烯氧化物环化酶提高黄花蒿中青蒿素的生物合成。

Overexpression of allene oxide cyclase improves the biosynthesis of artemisinin in Artemisia annua L.

机构信息

Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China.

Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.

出版信息

PLoS One. 2014 Mar 18;9(3):e91741. doi: 10.1371/journal.pone.0091741. eCollection 2014.

DOI:10.1371/journal.pone.0091741
PMID:24642483
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3958394/
Abstract

Jasmonates (JAs) are important signaling molecules in plants and play crucial roles in stress responses, secondary metabolites' regulation, plant growth and development. In this study, the promoter of AaAOC, which was the key gene of jasmonate biosynthetic pathway, had been cloned. GUS staining showed that AaAOC was expressed ubiquitiously in A. annua. AaAOC gene was overexpressed under control of 35S promoter. RT-Q-PCR showed that the expression levels of AaAOC were increased from 1.6- to 5.2-fold in AaAOC-overexpression transgenic A. annua. The results of GC-MS showed that the content of endogenous jasmonic acid (JA) was 2- to 4.7-fold of the control level in AaAOC-overexpression plants. HPLC showed that the contents of artemisinin, dihydroartemisinic acid and artemisinic acid were increased significantly in AaAOC-overexpression plants. RT-Q-PCR showed that the expression levels of FPS (farnesyl diphosphate synthase), CYP71AV1 (cytochrome P450 dependent hydroxylase) and DBR2 (double bond reductase 2) were increased significantly in AaAOC-overexpression plants. All data demonstrated that increased endogenous JA could significantly promote the biosynthesis of artemisinin in AaAOC-overexpression transgenic A. annua.

摘要

茉莉酸（JAs）是植物中重要的信号分子，在应激反应、次生代谢物调控、植物生长发育中发挥着关键作用。本研究克隆了参与茉莉酸生物合成途径的关键基因 AaAOC 的启动子。GUS 染色显示 AaAOC 在黄花蒿中广泛表达。在 35S 启动子的控制下，AaAOC 基因被过表达。RT-Q-PCR 显示 AaAOC 过表达转基因黄花蒿中 AaAOC 的表达水平增加了 1.6-5.2 倍。GC-MS 的结果表明，AaAOC 过表达植株中内源性茉莉酸（JA）的含量是对照水平的 2-4.7 倍。HPLC 显示 AaAOC 过表达植株中青蒿素、二氢青蒿酸和青蒿酸的含量显著增加。RT-Q-PCR 显示 AaAOC 过表达植株中 FPS（法呢基二磷酸合酶）、CYP71AV1（细胞色素 P450 依赖性羟化酶）和 DBR2（双键还原酶 2）的表达水平显著增加。所有数据表明，内源性 JA 的增加可显著促进 AaAOC 过表达转基因黄花蒿中青蒿素的生物合成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/b2f40db5ef29/pone.0091741.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/c7bb32f9a3ff/pone.0091741.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/71c9d5657f7d/pone.0091741.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/7b031507c97c/pone.0091741.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/c727dbd402b5/pone.0091741.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/df7e1443d650/pone.0091741.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/b2f40db5ef29/pone.0091741.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/c7bb32f9a3ff/pone.0091741.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/71c9d5657f7d/pone.0091741.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/7b031507c97c/pone.0091741.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/c727dbd402b5/pone.0091741.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/df7e1443d650/pone.0091741.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a02/3958394/b2f40db5ef29/pone.0091741.g006.jpg

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2
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Plant Cell Rep. 2012 Dec;31(12):2247-59. doi: 10.1007/s00299-012-1334-9. Epub 2012 Aug 29.
3
Characterization of a novel ERF transcription factor in Artemisia annua and its induction kinetics after hormones and stress treatments.
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Front Plant Sci. 2022 Aug 15;13:942789. doi: 10.3389/fpls.2022.942789. eCollection 2022.
4
Systemic Expression of Genes Involved in the Plant Defense Response Induced by Wounding in .系统表达植物防御反应相关基因在. 诱导的创伤中
Int J Mol Sci. 2021 Sep 17;22(18):10073. doi: 10.3390/ijms221810073.
5
Proteome Profiling of the Mutagen-Induced Morphological and Yield Macro-Mutant Lines of L.诱变诱导的番茄形态和产量宏观突变体系的蛋白质组分析
Plants (Basel). 2019 Sep 2;8(9):321. doi: 10.3390/plants8090321.
6
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Sci Adv. 2018 Nov 14;4(11):eaas9357. doi: 10.1126/sciadv.aas9357. eCollection 2018 Nov.
7
Overexpression of a cell wall damage induced transcription factor, OsWRKY42, leads to enhanced callose deposition and tolerance to salt stress but does not enhance tolerance to bacterial infection.过量表达细胞壁损伤诱导转录因子 OsWRKY42 导致胼胝质沉积增加和耐盐性增强，但不能增强对细菌感染的耐受性。
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Mycorrhiza. 2015 Jul;25(5):345-57. doi: 10.1007/s00572-014-0614-3. Epub 2014 Nov 5.
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4
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5
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6
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7
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Annu Rev Plant Biol. 2009;60:183-205. doi: 10.1146/annurev.arplant.043008.092007.
8
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