Asif Ambreen, K Ansari Mohammad Yunus, Hashem Abeer, Tabassum Baby, Abd Allah Elsayed Fathi, Ahmad Altaf
Department of Botany, Aligarh Muslim University, Aligarh 202002, India.
Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia.
Plants (Basel). 2019 Sep 2;8(9):321. doi: 10.3390/plants8090321.
In the present investigation, the leaf proteome profile of the macro-mutant lines of L. was analyzed to identify the key proteins involved in the expression of traits associated with the morphology, seed yield, and content of thymoquinone. In our earlier study, the macro-mutants were generated with contrasting morphological traits and seed yields through induced mutagenesis, using ethyl methyl sulfonate, gamma rays, and combinations of both. Analysis of the leaf proteome of the control and macro-mutant lines of showed that twenty-three proteins were differentially expressed. These differentially expressed proteins were sequenced through mass spectrometry and identified using the MASCOT software. On the basis of their function, these proteins were categorized into several groups. Most proteins were found in the categories of signal transduction (18%) and carbon metabolism (18%). A total of 13% of proteins belonged to the categories of energy and metabolism. Proteins in the categories of secondary plant metabolism, stress defense, cytoskeleton, and protein synthesis were also found. The polycomb group protein (FIE1), transcription factor (PRE1), and geranyl diphosphate synthase were notable proteins, in addition to some proteins of signal transduction and carbon metabolism. Expression patterns of the differentially expressed proteins were also studied at the transcript level by using qRT-PCR. Transcriptomics analysis was consistent with the proteomics data. This study shows the changes that take place at the proteomic level through induced mutagenesis, as well as the involvement of some proteins in the expression traits associated with plant height, seed yield, and the thymoquinone content of . The identified proteins might help elucidate the metabolic pathways involved in the expression of traits, including seed yield, and the active compounds of medicinal plants.
在本研究中，对罗勒的宏观突变体系的叶片蛋白质组图谱进行了分析，以鉴定与形态、种子产量和百里醌含量相关的性状表达中涉及的关键蛋白质。在我们早期的研究中，通过使用甲基磺酸乙酯、伽马射线以及两者的组合进行诱变，产生了具有不同形态性状和种子产量的宏观突变体。对罗勒对照和宏观突变体系的叶片蛋白质组分析表明，有23种蛋白质差异表达。这些差异表达的蛋白质通过质谱测序，并使用MASCOT软件进行鉴定。根据其功能，这些蛋白质被分为几个组。大多数蛋白质属于信号转导（18%）和碳代谢（18%）类别。共有13%的蛋白质属于能量和代谢类别。还发现了属于次生植物代谢、胁迫防御、细胞骨架和蛋白质合成类别的蛋白质。除了一些信号转导和碳代谢的蛋白质外，多梳蛋白组蛋白（FIE1）、转录因子（PRE1）和香叶基二磷酸合酶是值得注意的蛋白质。还通过使用qRT-PCR在转录水平上研究了差异表达蛋白质的表达模式。转录组学分析与蛋白质组学数据一致。本研究显示了通过诱变在蛋白质组水平上发生的变化，以及一些蛋白质在与罗勒株高、种子产量和百里醌含量相关的性状表达中的作用。所鉴定的蛋白质可能有助于阐明参与包括种子产量在内的性状表达以及药用植物活性化合物的代谢途径。
