Jiang Keji, Pi Yan, Hou Rong, Jiang Lili, Sun Xiaofen, Tang Kexuan
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Morgan-Tan International Center for Life Sciences, Fudan University, Shanghai, People's Republic of China.
Planta. 2009 Apr;229(5):1057-63. doi: 10.1007/s00425-009-0896-4. Epub 2009 Feb 12.
Plant secondary metabolites are a wide variety of low-molecular weight compounds whose productions are often enhanced in response to both biotic and abiotic stresses. Many of the responses are mediated by a class of hormones, named as jasmonates. In jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, EC 5.3.99.6) catalyzes the most crucial step. Here a heterologous AOC gene from Hyoscyamus niger L. (black henbane), named HnAOC (GenBank accession No. AY708383), was overexpressed in Nicotiana tabacum cv. Petit Havana to investigate the consequence on nicotine content. This study revealed that the transcription of HnAOC in tobacco resulted in overexpression of nicotine biosynthetic pathway genes and higher yield of nicotine, with the maximum of 4.8-fold over control. Therefore, it indicated that without the cost of extrinsic hormones, genetic manipulation of jasmonate biosynthetic pathway genes could be an alternative approach in metabolic engineering for the production of valuable secondary metabolites, which were induced by jasmonates.
植物次生代谢产物是多种低分子量化合物，其产量通常会因生物和非生物胁迫而增加。许多此类反应由一类名为茉莉酸酯的激素介导。在植物茉莉酸酯生物合成途径中，丙二烯氧化物环化酶（AOC，EC 5.3.99.6）催化最关键的步骤。在此，来自黑莨（Hyoscyamus niger L.）的一个异源AOC基因，命名为HnAOC（GenBank登录号AY708383），在烟草品种 Petit Havana中过表达，以研究其对尼古丁含量的影响。该研究表明，烟草中HnAOC的转录导致尼古丁生物合成途径基因的过表达和尼古丁产量的提高，最高比对照高4.8倍。因此，这表明在无需外源激素成本的情况下，对茉莉酸酯生物合成途径基因进行基因操作可能是代谢工程中生产由茉莉酸酯诱导的有价值次生代谢产物的一种替代方法。
