Cinco M, Banfi E, Panfili E
Istituto di Microbiologia, University of Trieste, Italy.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Nov;269(3):277-83. doi: 10.1016/s0176-6724(88)80171-8.
Purified preparations of lipopolysaccharides (LPS) extracted from two different strains of Leptospira interrogans have been electrophoretically analyzed in order to determine their location at the level of outer envelope (OE). Evidence has been collected for the presence of some LPS fractions in the OE, suggesting that a part of this molecule is embedded in the membrane structure. The serological specificity of the LPS has been in addition tested by means of monoclonal antiserovar antibodies (Moabs); the results indicated that the LPS structure is endowed of the immunodeterminants of the serovar. The remarkable relevance of this finding for the Leptospira taxonomy is discussed.
为了确定从两种不同问号钩端螺旋体菌株中提取的脂多糖（LPS）制剂在外膜（OE）水平的位置，对其进行了电泳分析。已收集到OE中存在一些LPS组分的证据，表明该分子的一部分嵌入膜结构中。此外，还通过单克隆抗血清型抗体（Moabs）测试了LPS的血清学特异性；结果表明，LPS结构具有血清型的免疫决定簇。讨论了这一发现对钩端螺旋体分类学的显著相关性。
