Cho S N, Uhm J R, Kim J D
Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Med J. 1992 Mar;33(1):24-31. doi: 10.3349/ymj.1992.33.1.24.
Lipopolysaccharide (LPS) or glycolipid antigens of Leptospira interrogans have been candidates as serogroup or serotype specific antigen. In this study, therefore, we prepared the LPS and lipid antigens from L. interrogans serovars lai, icterohaemorrhagiae, copenhageni, canicola, pomona, grippotyphosa, and a Korean isolate 30R. The LPS antigens were analyzed by a polyacrylamide gel electrophoresis and lipid antigens by thin-layer chromatography, respectively. The seroreactivity of the antigens were also examined with homologous or heterologous antisera using an enzyme-linked immunosorbent assay. The LPS antigens from serovar lai and the strain 30R were closely related but different from serovar icterohaemorrhagiae. Particularly, the LPS antigens from serovars icterohaemorrhagiae and grippotyphosa were reactive only with the homologous antisera, thus indicating serovar specificity. However, the LPS antigens of the other serovars were reactive to the heterologous antisera. The lipid antigen of serovar icterohaemorrhagiae reacted only with the homologous antisera. In contrast, lipids of other serovars reacted broadly with heterologous antisera, particularly among serovars lai, copenhageni, canicola, pomona, and the strain 30R. The results thus indicated that the LPS and lipid antigens of L. interrogans may contain serovar-specific as well as cross-reactive epitopes.
问号钩端螺旋体的脂多糖（LPS）或糖脂抗原一直是血清群或血清型特异性抗原的候选物质。因此，在本研究中，我们从问号钩端螺旋体赖型、黄疸出血型、哥本哈根型、犬型、波摩那型、七日热型血清型以及一株韩国分离株30R中制备了LPS和脂质抗原。分别通过聚丙烯酰胺凝胶电泳分析LPS抗原，通过薄层色谱分析脂质抗原。还使用酶联免疫吸附测定法用同源或异源抗血清检测了抗原的血清反应性。赖型血清型和30R菌株的LPS抗原密切相关，但与黄疸出血型血清型不同。特别是，黄疸出血型和七日热型血清型的LPS抗原仅与同源抗血清反应，因此表明具有血清型特异性。然而，其他血清型的LPS抗原与异源抗血清反应。黄疸出血型血清型的脂质抗原仅与同源抗血清反应。相比之下，其他血清型的脂质与异源抗血清广泛反应，特别是在赖型、哥本哈根型、犬型、波摩那型血清型和30R菌株之间。因此，结果表明问号钩端螺旋体的LPS和脂质抗原可能包含血清型特异性以及交叉反应性表位。
