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[红细胞葡萄球菌诊断剂对致敏素制剂中蛋白A存在情况的特异性]

[The specificity of erythrocyte staphylococcal diagnostic agents in relation to the presence of protein A in sensitin preparations].

作者信息

Deriabin P N, Karal'nik B V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1988 Nov(11):85-9.

PMID:2464263
Abstract

Protein A has been detected in Staphylococcus aureus muriatic extracts by means of the passive hemagglutination test and the passive hemagglutination inhibition test with IgG erythrocyte diagnosticum. The absence of perceptible amounts of protein A, capable of interaction with the Fc fragment of IgG, on the surface of erythrocyte diagnosticum obtained from S. aureus muriatic extract with the use of amidol has been proved by different methods. The use of antierythrocytic IgG antibody bridge has shown that protein A in S. aureus muriatic extract is bound with other species-specific antigens, in particular with teichoic acid.

摘要

通过被动血凝试验和使用IgG红细胞诊断剂的被动血凝抑制试验,在金黄色葡萄球菌盐酸提取物中检测到了蛋白A。已通过不同方法证明,在用氨基醇从金黄色葡萄球菌盐酸提取物中获得的红细胞诊断剂表面,不存在能够与IgG的Fc片段相互作用的可察觉量的蛋白A。使用抗红细胞IgG抗体桥已表明,金黄色葡萄球菌盐酸提取物中的蛋白A与其他种特异性抗原结合,特别是与磷壁酸结合。

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