Koito A, Hattori T, Matsushita S, Maeda Y, Nozaki C, Sagawa K, Takatsuki K
Second Department of Internal Medicine, Kumamoto University Medical School, Japan.
AIDS Res Hum Retroviruses. 1988 Dec;4(6):409-17. doi: 10.1089/aid.1988.4.409.
A murine monoclonal antibody (MoAb), VAK 4, has been known to specifically react with a major core protein (p24) as well as with its precursor (p55-57) and intermediate precursor (p40) of human immunodeficiency virus strain IIIB (HTLV-IIIB). Radioimmunoprecipitation assays revealed that VAK 4 MoAb precipitated a major core protein and its precursors from a variety of strains of HIV and also from simian immunodeficiency virus (SIV), although the molecular weights of the precursor proteins in each viral strain were slightly different. A protein synthesized by transfected Escherichia coli containing amino acid sequences corresponding to residues 121-436 of the HTLV-IIIB gag gene was reactive with VAK 4 MoAb, but the protein carrying only residues 121-309 was not reactive, suggesting that the epitope recognized by VAK 4 MoAb resides at the carboxyl terminus of p24 protein. A competitive enzyme-linked immunosorbent assay showed that patient sera containing anticore protein antibody inhibited the binding of VAK 4 to HTLV-IIIB. These findings suggested that VAK 4 MoAb recognized an immunogenic and conserved epitope belonging to a major core protein of HIV-related viruses.
已知一种鼠单克隆抗体（MoAb）VAK 4能与人免疫缺陷病毒IIIB株（HTLV-IIIB）的一种主要核心蛋白（p24）及其前体（p55 - 57）和中间前体（p40）发生特异性反应。放射免疫沉淀分析表明，VAK 4单克隆抗体能从多种HIV毒株以及猿猴免疫缺陷病毒（SIV）中沉淀出一种主要核心蛋白及其前体，尽管每种病毒株中前体蛋白的分子量略有不同。由转染的大肠杆菌合成的、含有与HTLV-IIIB gag基因第121 - 436位残基对应的氨基酸序列的蛋白质能与VAK 4单克隆抗体发生反应，但仅携带第121 - 309位残基的蛋白质无反应，这表明VAK 4单克隆抗体识别的表位位于p24蛋白的羧基末端。竞争性酶联免疫吸附试验表明，含有抗核心蛋白抗体的患者血清能抑制VAK 4与HTLV-IIIB的结合。这些发现提示，VAK 4单克隆抗体识别的是属于HIV相关病毒主要核心蛋白的一个免疫原性保守表位。
