Ghrayeb J, Kato I, McKinney S, Huang J J, Chanda P K, Ho D D, Sarangadharan M G, Chang T W, Chang N T
DNA. 1986 Apr;5(2):93-9. doi: 10.1089/dna.1986.5.93.
Fragments of human T-cell lymphotropic virus type III (HTLV-III) proviral DNA carrying the gene for the core antigen (gag) was cloned in the plasmid REV. Several of the recombinants direct high levels of synthesis of the antigens. One clone, pG1, produced a hybrid protein containing 13 amino acid residues of the carboxyl terminus of the 17 kD virion protein, the entire p24, the major core protein of HTLV-III, and 74 amino acid residues of the amino terminal of the 15 kD core ribonucleoprotein. A second clone, pG2, was similar to pG1 except that it contained no p17 sequences and was missing the amino-terminal 77 amino acid residues of the p24. A third clone, pG3, was similar to pG2, except that all but 56 amino acids of the carboxyl terminus of p24 were removed. All three proteins were found to be strongly immunoreactive with anti-HTLV-III antibodies present in sera from patients with acquired immune deficiency syndrome (AIDS) or AIDS-related complex (ARC). In addition, pG1 and pG2, but not pG3, reacted with a monoclonal antibody (M26) specific for the p24 virion core protein. Whereas all three reacted with an anti-p15 monoclonal antibody, none of the clones reacted with an anti-p17 monoclonal antibody. These results provide direct evidence to support the predicted assignment of the coding region of the gag gene of HTLV-III. The product from pG2 was purified and was found to be potentially useful for the detection of anti-p24 antibodies in sera from patients with AIDS or ARC and from individuals at risk from AIDS.
携带核心抗原（gag）基因的人类III型嗜T细胞病毒（HTLV-III）前病毒DNA片段被克隆到质粒REV中。几个重组体指导高水平的抗原合成。一个克隆体pG1产生一种杂合蛋白，该蛋白包含17kD病毒粒子蛋白羧基末端的13个氨基酸残基、完整的p24（HTLV-III的主要核心蛋白）以及15kD核心核糖核蛋白氨基末端的74个氨基酸残基。第二个克隆体pG2与pG1相似，只是它不包含p17序列且缺少p24氨基末端的77个氨基酸残基。第三个克隆体pG3与pG2相似，只是p24羧基末端除56个氨基酸外其余都被去除。发现所有这三种蛋白与获得性免疫缺陷综合征（AIDS）或AIDS相关综合征（ARC）患者血清中存在的抗HTLV-III抗体有强烈的免疫反应性。此外，pG1和pG2能与针对p24病毒粒子核心蛋白的单克隆抗体（M26）发生反应，但pG3不能。虽然所有三个克隆体都能与抗p15单克隆抗体发生反应，但没有一个克隆体与抗p17单克隆抗体发生反应。这些结果提供了直接证据支持HTLV-III gag基因编码区的预测分配。来自pG2的产物被纯化，发现它可能有助于检测AIDS或ARC患者以及有AIDS风险个体血清中的抗p24抗体。
