Environ Technol. 2014 Mar-Apr;35(5-8):1012-7. doi: 10.1080/09593330.2013.858755.
A simple and inexpensive biosensor based on lyophilized mushroom tissue (Agaricus bisporus) was developed for amperometric determination of phenol. This fungi tissue contains tyrosinase (EC 1.14.18.1) enzyme that catalysis two sequential oxidation reactions with phenolic substrates. Both reactions involve molecular oxygen; therefore, the commercial Clark-type oxygen electrode was selected as a transducer. The lyophilized biocomponent was tested in two different forms: cubes (at two positions in the biosensor system) or powder. In characterization studies of the biosensor, some parameters such as time reaction, linear range and repeatability were investigated. For the best biosensor configuration, a linear response was observed from 0.1 to 10.0mg L(-1) phenol; variation coefficient and standard deviation were calculated as 0.02% and +/- 0.11mg L(-1), respectively.
基于冻干蘑菇组织（双孢蘑菇）的简单廉价生物传感器已被开发用于苯酚的电流测定法。这种真菌组织含有酪氨酸酶（EC 1.14.18.1）酶，该酶可催化酚类底物的两个连续氧化反应。两个反应都涉及分子氧；因此，选择了商业克拉克型氧电极作为传感器。冻干生物组件以两种不同形式进行了测试：立方体（在生物传感器系统的两个位置）或粉末。在传感器的特性研究中，研究了一些参数，例如反应时间、线性范围和重复性。对于最佳生物传感器配置，从 0.1 到 10.0mg L(-1)苯酚观察到线性响应；变异系数和标准偏差分别计算为 0.02%和 +/- 0.11mg L(-1)。
