Department of Biochemical Engineering, Federal University of Rio de Janeiro--UFRJ, School of Chemistry, Rio de Janeiro, Brazil.
Environ Technol. 2010 May;31(6):611-6. doi: 10.1080/09593331003592238.
Phenols are toxic compounds that are present in several industrial wastewaters, so their quantification has great environmental importance. In order to permit an analytical methodology for in situ monitoring, this work aims to study the application of Agaricus bisporus tissue as a source of tyrosinase and the optimum reaction conditions for the development of a phenol biosensor. Such an enzyme is a polyphenol oxidase that transforms many different phenolic compounds into quinones. Experiments with fungi tissue were performed to evaluate different sizes of tissue (0.5, 1.0 and 1.5 cm), different temperatures (23.5 degrees C to 60 degrees C), and different pH values (6, 7 and 8) to quantify analytically phenol content. Amongst the tested conditions, those that had presented larger efficiency in phenol oxidation were attained with the fungal tissue size of 1 cm, at pH 8.0, in the temperature range from 35 degrees C to 45 degrees C.
酚类是存在于几种工业废水中的有毒化合物，因此它们的定量具有重要的环境意义。为了允许进行原位监测的分析方法，本工作旨在研究双孢蘑菇组织作为酪氨酸酶来源的应用，并确定开发酚类生物传感器的最佳反应条件。这种酶是多酚氧化酶，它将许多不同的酚类化合物转化为醌。通过真菌组织实验评估了不同大小的组织（0.5、1.0 和 1.5 cm）、不同温度（23.5°C 至 60°C）和不同 pH 值（6、7 和 8）对酚类含量进行分析的情况。在所测试的条件中，在 pH 值为 8.0、温度范围为 35°C 至 45°C 时，真菌组织尺寸为 1 cm 时，酚类氧化的效率最大。
