Department of Biochemical Engineering, School of Chemistry, Federal University of Rio de Janeiro (UFRJ), E-122, University City, CEP 21949-909, Rio de Janeiro, Brazil.
Environ Technol. 2011 Apr;32(5-6):493-7. doi: 10.1080/09593330.2010.504234.
With the aim of searching for an in situ method for monitoring phenol, Agaricus bisporus tissue with tyrosine activity was used as a biocomponent and an oxygen electrode used as a transducer to develop a biosensor. The experimental methodology investigated the relation between dissolved oxygen and phenol concentration using a standard solution. Biosensor calibration was evaluated by studying reaction time and tissue amount necessary to promote a reliable response and to minimize errors. The influence of air saturation of the sample and washing of the electrode was also investigated. Results showed that 5 g of mushroom tissue with a 1 min reaction time promoted the best biosensor response within a phenol concentration range of 5-10 ppm. Washing of the electrode did not change the performance of the analysis; however, initial air saturation caused less variation amongst the samples.
为了寻找一种原位监测苯酚的方法，本研究以具有酪氨酸活性的双孢蘑菇组织作为生物成分，利用氧电极作为传感器，开发了一种生物传感器。实验方法采用标准溶液研究了溶解氧与苯酚浓度之间的关系。通过研究促进可靠响应和最小化误差所需的反应时间和组织量来评估生物传感器的校准。还研究了样品的空气饱和和电极清洗的影响。结果表明，在 5-10ppm 苯酚浓度范围内，使用 5g 蘑菇组织和 1 分钟的反应时间可获得最佳的生物传感器响应。电极的清洗不会改变分析的性能；然而，初始空气饱和会减少样品之间的差异。
