一种用于植物组织杂交组织化学的简单固定和包埋方法。
A simple fixation and embedding method for use in hybridization histochemistry on plant tissues.
作者信息
McFadden G I, Bonig I, Cornish E C, Clarke A E
机构信息
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
出版信息
Histochem J. 1988 Oct;20(10):575-86. doi: 10.1007/BF01002612.
A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.
已开发出一种用于杂交组织化学的植物组织固定和包埋的简单方法。材料通过相分离技术用多聚甲醛-戊二醛固定，并包埋在LR Gold中。该方法可原位保存核酸。双链32P标记的DNA探针与薄切片和半薄切片中的互补RNA杂交。用免疫金标记检测的生物素化探针已用于在电子显微镜水平定位转录本。
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