Yang Fan, Zheng Tianpeng, Gao Yun, Baskota Attit, Chen Tao, Ran Xingwu, Tian Haoming
Department of Endocrinology and Metabolism, West China Hospital of Sichuan University, Sichuan, P. R. China; Department of Endocrinology and Metabolism, Affiliated Hospital of Guilin Medical University, Guangxi, P. R. China.
Department of Endocrinology and Metabolism, West China Hospital of Sichuan University, Sichuan, P. R. China.
PLoS One. 2014 Mar 19;9(3):e92222. doi: 10.1371/journal.pone.0092222. eCollection 2014.
To determine whether fasting plasma Dipeptidyl Peptidase 4 (DPP4) activity and active Glucagon-Like Peptide-1 (GLP-1) were predictive of the onset of metabolic syndrome.
A prospective cohort study was conducted of 2042 adults (863 men and 1,179 women) aged 18-70 years without metabolic syndrome examined in 2007(baseline) and 2011(follow-up). Baseline plasma DPP4 activity was determined as the rate of cleavage of 7-amino-4- methylcoumarin (AMC) from the synthetic substrate H-glycyl-prolyl-AMC and active GLP-1 was determined by enzymoimmunoassay.
During an average of 4 years of follow-up, 131 men (15.2%) and 174 women (14.8%) developed metabolic syndrome. In multiple linear regression analysis, baseline DPP4 activity was an independent predictor of an increase in insulin resistance over a 4-year period (P<0.01). In multivariable-adjusted models, the odds ratio (OR) for incident metabolic syndrome comparing the highest with the lowest quartiles of DPP4 activity and active GLP-1 were 2.82, 0.45 for men and 2.48, 0.36 for women respectively. Furthermore, plasma DPP4 activity significantly improved the area under the ROC curve for predicting new-onset metabolic syndrome based on information from metabolic syndrome components (Both P<0.01).
DPP4 activity is an important predictor of the onset of insulin resistance and metabolic syndrome in apparently healthy Chinese men and women. This finding may have important implications for understanding the aetiology of metabolic syndrome.
#TR-CCH-Chi CTR-CCH-00000361.
确定空腹血浆二肽基肽酶4（DPP4）活性和活性胰高血糖素样肽-1（GLP-1）是否可预测代谢综合征的发生。
对2042名年龄在18 - 70岁、无代谢综合征的成年人（863名男性和1179名女性）进行了一项前瞻性队列研究，于2007年（基线）和2011年（随访）进行检查。基线血浆DPP4活性通过测定从合成底物H - 甘氨酰 - 脯氨酰 - 7 - 氨基 - 4 - 甲基香豆素（AMC）中裂解出7 - 氨基 - 4 - 甲基香豆素（AMC）的速率来确定，活性GLP - 1通过酶免疫测定法测定。
在平均4年的随访期间，131名男性（15.2%）和174名女性（14.8%）患上了代谢综合征。在多元线性回归分析中，基线DPP4活性是4年内胰岛素抵抗增加的独立预测因素（P<0.01）。在多变量调整模型中，将DPP4活性和活性GLP - 1的最高四分位数与最低四分位数相比，男性发生代谢综合征的比值比（OR）分别为2.82、0.45，女性分别为2.48、0.36。此外，基于代谢综合征各组分的信息，血浆DPP4活性显著改善了预测新发代谢综合征的ROC曲线下面积（P均<0.01）。
DPP4活性是中国健康男性和女性中胰岛素抵抗和代谢综合征发生的重要预测因素。这一发现可能对理解代谢综合征的病因具有重要意义。
#TR - CCH - Chi CTR - CCH - 00000361。
