Sugimoto Naotoshi, Shido Osamu, Matsuzaki Kentaro, Katakura Masanori, Hitomi Yoshiaki, Tanaka Masao, Sawaki Toshioki, Fujita Yoshimasa, Kawanami Takafumi, Masaki Yasufumi, Okazaki Toshiro, Nakamura Hiroyuki, Koizumi Shoichi, Yachie Akihiro, Umehara Hisanori
Department of Physiology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8640, Japan,
Cell Biochem Biophys. 2014 Sep;70(1):301-7. doi: 10.1007/s12013-014-9912-9.
Long-term continuous exposure to high ambient temperatures induces complete heat acclimation in humans and animals. However, to date, the effects of long-term exposure to heat stress on cells have not been fully evaluated. In this study, we investigated an adaptive physiological process induced in culture cells by continuous exposure to mild heat stress for 60 days. The results of this investigation provide evidence that after long-term heat acclimation in cells, (1) heat shock protein levels are increased, (2) hypoxia inducible factor-1α (HIF-1α) expression is upregulated, and (3) heat shock-induced and hypoxia-induced apoptoses are attenuated. These results suggest that the hypoxia response pathway is an intrinsic part of the heat acclimation repertoire and that the HIF-1 pathway following long-term heat acclimation induces cells with cross tolerance against hypoxia.
长期持续暴露于高温环境会使人类和动物实现完全的热适应。然而，迄今为止，长期暴露于热应激对细胞的影响尚未得到充分评估。在本研究中，我们调查了培养细胞连续暴露于轻度热应激60天所诱导的适应性生理过程。该调查结果表明，细胞长期热适应后，（1）热休克蛋白水平升高，（2）缺氧诱导因子-1α（HIF-1α）表达上调，（3）热休克诱导的凋亡和缺氧诱导的凋亡均减弱。这些结果表明，缺氧反应途径是热适应机制的固有组成部分，长期热适应后的HIF-1途径可诱导细胞产生对缺氧的交叉耐受性。
