Li Chenyu, Xie Ruxin, Zhang Shiwei, Yun Jiao, Zhao Tian, Zhong Ai, Zhang Jinjue, Chen Junjie
Department of Plastic and Burn Surgery, West China Hospital, Sichuan University, No. 37 Guoxue Alley, Wuhou District, Chengdu, 610041, Sichuan, China.
Arch Dermatol Res. 2025 Jan 8;317(1):204. doi: 10.1007/s00403-024-03747-x.
The stress response following burns may be a crucial factor in keloid formation, yet the underlying pathological mechanisms remain to be elucidated. This study initially investigated how heat shock factor 1 (HSF1) and heat shock proteins (HSPs) within the heat shock pathway influence keloid fibrosis, providing insights into the role of the heat shock response in keloid development. This study aims to further elucidate the role of the heat shock pathway in keloid fibrosis and investigate the specific function of HSF1 within this pathway. This study focused on human keloid fibroblasts, examining the expression and regulatory role of HSF1 on HSPs under heat stress using immunohistochemistry, RNA interference, real-time fluorescent PCR, and Western blotting techniques. HSF1 was overexpressed in keloid fibroblasts and tissues compared to normal skin, and heat stress could further enhance HSF1 expression in both keloid tissues and fibroblasts. Functional inhibition of HSF1 significantly affected the expression of downstream HSPs in keloid fibroblasts, ultimately leading to the inhibition of keloid fibrosis. The heat shock pathway plays a crucial role in keloid fibrosis, with HSF1 as a key regulator influencing the expression of HSPs. Heat stress treatment of keloid fibroblasts offers an approach for investigating keloid formation.
烧伤后的应激反应可能是瘢痕疙瘩形成的关键因素，但其潜在的病理机制仍有待阐明。本研究最初探讨了热休克途径中的热休克因子1（HSF1）和热休克蛋白（HSPs）如何影响瘢痕疙瘩纤维化，从而深入了解热休克反应在瘢痕疙瘩发展中的作用。本研究旨在进一步阐明热休克途径在瘢痕疙瘩纤维化中的作用，并研究HSF1在该途径中的具体功能。本研究聚焦于人瘢痕疙瘩成纤维细胞，运用免疫组织化学、RNA干扰、实时荧光定量PCR和蛋白质印迹技术，检测热应激条件下HSF1对HSPs的表达及调控作用。与正常皮肤相比，HSF1在瘢痕疙瘩成纤维细胞和组织中过表达，热应激可进一步增强瘢痕疙瘩组织和成纤维细胞中HSF1的表达。对HSF1进行功能抑制可显著影响瘢痕疙瘩成纤维细胞中下游HSPs的表达，最终导致瘢痕疙瘩纤维化受到抑制。热休克途径在瘢痕疙瘩纤维化中起关键作用，HSF1作为关键调节因子影响HSPs的表达。对瘢痕疙瘩成纤维细胞进行热应激处理为研究瘢痕疙瘩形成提供了一种方法。
