Division of Gastroenterology and Hepatology, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
J Virol. 2014 Jun;88(11):6519-23. doi: 10.1128/JVI.00224-14. Epub 2014 Mar 19.
Hepatitis C virus (HCV) nonstructural protein 2 (NS2) is required for HCV polyprotein processing and particle assembly. It comprises an N-terminal membrane domain and a C-terminal, cytosolically oriented protease domain. Here, we demonstrate that the NS2 protease domain itself associates with cellular membranes. A single charged residue in the second α-helix of the NS2 protease domain is required for proper membrane association, NS2 protein stability, and efficient HCV polyprotein processing.
丙型肝炎病毒 (HCV) 非结构蛋白 2 (NS2) 是 HCV 多蛋白加工和颗粒组装所必需的。它包含一个 N 端膜结构域和一个 C 端胞质定位的蛋白酶结构域。在这里，我们证明 NS2 蛋白酶结构域本身与细胞膜结合。NS2 蛋白酶结构域中第二个α螺旋中的一个单一电荷残基对于正确的膜结合、NS2 蛋白稳定性和高效 HCV 多蛋白加工是必需的。
