原人参二醇型人参皂苷对人结肠癌细胞的抗增殖作用。
Antiproliferative effects of protopanaxadiol ginsenosides on human colorectal cancer cells.
作者信息
Zheng Yan, Nan Hongmei, Hao Miao, Song Chengcheng, Zhou Yifa, Gao Yufei
机构信息
China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, P.R. China.
The First Affiliated Hospital to Changchun University of Chinese Medicine, Changchun, Jilin 130021, P.R. China.
出版信息
Biomed Rep. 2013 Jul;1(4):555-558. doi: 10.3892/br.2013.104. Epub 2013 May 10.
Abstract
Ginsenosides are the main biologically active components of ginseng. In this study, seven types of protopanaxadiol ginsenosides were assessed for their antiproliferative activity on the HCT-116 and HT-29 human colorectal cancer cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The experimental results indicated that the native protopanaxadiol ginsenosides Rb1 and Rb2 inhibited the proliferation of the colorectal cancer cells in a dose-dependent manner. The deglycosylation products F2 and CO (from ginsenosides Rb1 and Rb2, respectively) significantly inhibited the growth of the human colorectal cancer cell lines, whereas product C-K (from Rb1 and Rb2) exerted no antiproliferative effects on the cancer cell lines assessed in this study. HT-29 cells were more sensitive to these ginsenosides compared to HCT-116 cells. In addition, the antiproliferative activity of ginsenosides was found to be correlated with the number and type of sugar residues. The potent growth inhibitory effect of protopanaxadiol ginsenosides on cancer cells may be used in the pharmaceutical industry.
摘要
人参皂苷是人参的主要生物活性成分。在本研究中，使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法评估了七种原人参二醇型人参皂苷对HCT-116和HT-29人结肠癌细胞系的抗增殖活性。实验结果表明，天然原人参二醇型人参皂苷Rb1和Rb2以剂量依赖性方式抑制结肠癌细胞的增殖。去糖基化产物F2和CO（分别来自人参皂苷Rb1和Rb2）显著抑制人结肠癌细胞系的生长，而产物C-K（来自Rb1和Rb2）对本研究中评估的癌细胞系没有抗增殖作用。与HCT-116细胞相比，HT-29细胞对这些人参皂苷更敏感。此外，发现人参皂苷的抗增殖活性与糖残基的数量和类型相关。原人参二醇型人参皂苷对癌细胞的强效生长抑制作用可用于制药行业。
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