Ketring J L, Davis J J, Feeback D L, Leech R W, Brumback R A
Department of Pathology, University of Oklahoma College of Medicine, Oklahoma City.
Arch Pathol Lab Med. 1989 Feb;113(2):196-8.
The modified Bielschowsky stain has become the standard silver impregnation technique for use in paraffin-embedded tissue sections for identifying abnormalities of neuritic processes in a variety of neurodegenerative disorders such as Alzheimer's disease. Recently, glycol methacrylate embedding has been used increasingly as a substitute for paraffin embedding of tissue, because it results in less tissue distortion during processing and provides greater clarity and resolution of cellular architecture and structural details. We have been able to modify the Bielschowsky stain for use in glycol methacrylate-embedded brain sections. The use of this technique will permit more accurate quantitation of the histopathologic changes in neuritic processes in neurodegenerative diseases.
改良的 Bielschowsky 染色已成为用于石蜡包埋组织切片的标准银浸染技术，用于识别多种神经退行性疾病（如阿尔茨海默病）中神经突的异常。最近，甲基丙烯酸乙二醇酯包埋越来越多地被用作组织石蜡包埋的替代方法，因为它在处理过程中导致的组织变形较小，并能更清晰地显示细胞结构和结构细节。我们已经能够对 Bielschowsky 染色进行改良，以用于甲基丙烯酸乙二醇酯包埋的脑切片。这项技术的应用将使神经退行性疾病中神经突的组织病理学变化的定量更加准确。
