Schwartz D A, Munger R, Lazzaro B, Lumb G
Department of Pathology and Laboratory Medicine, Hahnemann University School of Medicine, Philadelphia.
Arch Pathol Lab Med. 1989 Feb;113(2):181-3.
Plastic embedding is a recently developed technique that has been shown to be superior to conventional paraffin embedding in the histopathologic identification of microorganisms in tissue specimens. This report describes a modification of the Brown and Hopps stain for the differentiation of gram-positive and gram-negative microorganisms in 3-micron-thick plastic sections. This technique is easily performed and has a rapid turnaround time. This staining method permits a more precise histopathologic diagnosis of infectious agents than is possible in paraffin-embedded tissues.
塑料包埋是一项最近开发的技术，在组织标本中微生物的组织病理学鉴定方面，已证明它优于传统的石蜡包埋。本报告描述了对Brown和Hopps染色法的一种改良，用于在3微米厚的塑料切片中鉴别革兰氏阳性和革兰氏阴性微生物。该技术操作简便，周转时间短。与石蜡包埋组织相比，这种染色方法能对感染因子做出更精确的组织病理学诊断。
