Sarasquete M C, Gonzalez de Canales M L
Instituto de Ciencias Marinas de Andalucía, Cádiz, Spain.
Eur J Histochem. 1993;37(3):267-72.
Semithin sections of different tissues of Seriola dumerili R. Embedded in glycol methacrylate, were stained with two staining methods normally used in conventional haematology and histology: Gutierrez's cytopanchrome and Gutierrez's polychrome V.O.F. The results observed in all tissues embedded in this resin and stained with V.O.F. are satisfactory and comparable, as to staining affinity, to those obtained on paraffin-embedded tissues. Gutierrez's trichrome stain appears to be a useful tool in the study of oogenesis for detecting changes in staining according to cellular structure and maturation stage. The results observed with cytopanchrome are similar to those obtained with toluidine blue in glycol methacrylate-embedded tissues. The use of these two staining techniques, requires no previous oxidation, alkali treatments, high temperatures or removal of the embedding medium. Furthermore, both dyes can be used at the same time: in this case, cytoplasm of previtellogenic oocytes showed a stronger affinity for cytopanchrome, and were more intensely stained by V.O.F. as the vitellogenesis of Seriola dumerili progressed. Both stain, and especially the cytopanchrome one, also are available for staining the liver, gills and testis.
杜氏鰤不同组织的半薄切片包埋于乙二醇甲基丙烯酸酯中，采用常规血液学和组织学中常用的两种染色方法进行染色：古铁雷斯细胞全色染色法和古铁雷斯多色V.O.F染色法。用V.O.F对包埋于这种树脂中的所有组织进行染色，观察到的结果在染色亲和力方面令人满意，且与石蜡包埋组织所获得的结果具有可比性。古铁雷斯三色染色法似乎是研究卵子发生的一种有用工具，可根据细胞结构和成熟阶段检测染色变化。在乙二醇甲基丙烯酸酯包埋组织中，细胞全色染色法观察到的结果与甲苯胺蓝染色法相似。使用这两种染色技术无需预先氧化、碱处理、高温或去除包埋介质。此外，两种染料可同时使用：在这种情况下，随着杜氏鰤卵黄发生的进展，卵黄发生前的卵母细胞的细胞质对细胞全色染色法显示出更强的亲和力，并且被V.O.F染色更深。这两种染料，尤其是细胞全色染料，也可用于肝脏、鳃和睾丸的染色。
