Driscoll Meghan K, Sun Xiaoyu, Guven Can, Fourkas John T, Losert Wolfgang
Department of Physics, ‡Department of Chemistry and Biochemistry, and §Institute for Physical Science and Technology, University of Maryland , College Park, Maryland, United States.
ACS Nano. 2014 Apr 22;8(4):3546-55. doi: 10.1021/nn406637c. Epub 2014 Mar 27.
We investigate the effects of surface nanotopography on the migration and cell shape dynamics of the amoeba Dictyostelium discoideum. Multiple prior studies have implicated the patterning of focal adhesions in contact guidance. However, we observe significant contact guidance of Dictyostelium along surfaces with nanoscale ridges or grooves, even though this organism lacks integrin-based adhesions. Cells that move parallel to nanoridges are faster, more protrusive at their fronts, and more elongated than are cells that move perpendicular to nanoridges. Quantitative studies show that nanoridges spaced 1.5 μm apart exhibit the greatest contact guidance efficiency. Because Dictyostelium cells exhibit oscillatory shape dynamics, we model contact guidance as a process in which stochastic cellular harmonic oscillators couple to the periodicity of the nanoridges. In support of this connection, we find that nanoridges nucleate actin polymerization waves of nanoscale width that propagate parallel to the nanoridges.
我们研究了表面纳米形貌对盘基网柄菌变形虫迁移和细胞形态动力学的影响。先前的多项研究表明，粘着斑的模式在接触导向中起作用。然而，我们观察到盘基网柄菌沿着具有纳米级脊或凹槽的表面有显著的接触导向，尽管这种生物体缺乏基于整合素的粘附。与纳米脊平行移动的细胞比垂直于纳米脊移动的细胞速度更快，前端更突出，且更细长。定量研究表明，间距为1.5μm的纳米脊表现出最大的接触导向效率。由于盘基网柄菌细胞表现出振荡的形态动力学，我们将接触导向建模为一个随机细胞谐波振荡器与纳米脊的周期性耦合的过程。为支持这种联系，我们发现纳米脊引发了与纳米脊平行传播的纳米级宽度的肌动蛋白聚合波。
