Bettinger Christopher J, Langer Robert, Borenstein Jeffrey T
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
Angew Chem Int Ed Engl. 2009;48(30):5406-15. doi: 10.1002/anie.200805179.
The interaction of mammalian cells with nanoscale topography has proven to be an important signaling modality in controlling cell function. Naturally occurring nanotopographic structures within the extracellular matrix present surrounding cells with mechanotransductive cues that influence local migration, cell polarization, and other functions. Synthetically nanofabricated topography can also influence cell morphology, alignment, adhesion, migration, proliferation, and cytoskeleton organization. We review the use of in vitro synthetic cell-nanotopography interactions to control cell behavior and influence complex cellular processes, including stem-cell differentiation and tissue organization. Future challenges and opportunities in cell-nanotopography engineering are also discussed, including the elucidation of mechanisms and applications in tissue engineering.
哺乳动物细胞与纳米级形貌的相互作用已被证明是控制细胞功能的一种重要信号传导方式。细胞外基质中天然存在的纳米级形貌结构为周围细胞提供了机械转导线索，影响局部迁移、细胞极化和其他功能。人工合成的纳米制造形貌也会影响细胞形态、排列、黏附、迁移、增殖和细胞骨架组织。我们综述了利用体外合成的细胞与纳米形貌相互作用来控制细胞行为并影响复杂细胞过程，包括干细胞分化和组织构建。还讨论了细胞与纳米形貌工程未来面临的挑战和机遇，包括作用机制的阐明及其在组织工程中的应用。
