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1
TMA-DPH a fluorescent probe of membrane dynamics in living cells. How to use it in phagocytosis.
Cell Biophys. 1989 Feb;14(1):17-26. doi: 10.1007/BF02797388.
2
Internalization of the lipophilic fluorescent probe trimethylamino-diphenylhexatriene follows the endocytosis and recycling of the plasma membrane in cells.亲脂性荧光探针三甲氨基二苯基己三烯的内化过程遵循细胞中质膜的内吞作用和再循环过程。
Biochim Biophys Acta. 1990 Nov 30;1030(1):73-81. doi: 10.1016/0005-2736(90)90240-o.
3
A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane.将TMA-DPH作为质膜和内吞膜探针时的荧光特性比较。
Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66. doi: 10.1016/0005-2736(95)00135-p.
4
TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells.TMA-DPH：一种适用于完整活细胞中特定质膜流动性研究的荧光偏振探针。
Cell Biophys. 1983 Jun;5(2):129-40. doi: 10.1007/BF02796139.
5
The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells.用TMA-DPH进行细胞内荧光标记的动力学方面支持L929细胞内吞作用的成熟模型。
J Cell Biol. 1994 May;125(4):783-94. doi: 10.1083/jcb.125.4.783.
6
Parallel investigation of exocytosis kinetics and membrane fluidity changes in human platelets with the fluorescent probe, trimethylammonio-diphenylhexatriene.使用荧光探针三甲基铵-二苯基己三烯对人血小板胞吐动力学和膜流动性变化进行平行研究。
Biochim Biophys Acta. 1987 Jul 10;901(1):138-46. doi: 10.1016/0005-2736(87)90265-3.
7
Bidirectional transbilayer lipid movement in human platelets as vizualized by the fluorescent membrane probe 1-[4-(trimethylammonio)phenyl]-6-phenyl-1,3,5-hexatriene.用荧光膜探针1-[4-(三甲基铵基)苯基]-6-苯基-1,3,5-己三烯观察到的人血小板中双向跨双层脂质运动。
Biochemistry. 1990 May 29;29(21):5132-7. doi: 10.1021/bi00473a019.
8
Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdiphenylhexatriene.通过三甲基铵二苯基己三烯的荧光各向异性对完整活细胞进行质膜流动性测量。
Biochim Biophys Acta. 1985 Apr 22;845(1):60-7. doi: 10.1016/0167-4889(85)90055-2.
9
The plasma-membrane internalization and recycling is enhanced in macrophages upon activation with gamma-interferon and lipopolysaccharide; a study using the fluorescent probe trimethylaminodiphenylhexatriene.在用γ-干扰素和脂多糖激活后，巨噬细胞中的质膜内化和再循环增强；一项使用荧光探针三甲基氨基二苯基己三烯的研究。
Biochim Biophys Acta. 1990 Nov 30;1030(1):82-7. doi: 10.1016/0005-2736(90)90241-f.
10
Differentiation between clathrin-dependent and clathrin-independent endocytosis by means of membrane fluidity measurements.通过膜流动性测量区分网格蛋白依赖的内吞作用和非网格蛋白依赖的内吞作用。
Cell Biochem Biophys. 1999;30(1):25-34. doi: 10.1007/BF02737883.

引用本文的文献

1
P2X7-mediated alteration of membrane fluidity is associated with the late stages of age-related macular degeneration.P2X7 介导的膜流动性改变与年龄相关性黄斑变性的晚期阶段有关。
Purinergic Signal. 2022 Dec;18(4):469-479. doi: 10.1007/s11302-022-09894-y. Epub 2022 Aug 24.

本文引用的文献

1
Non-specific binding by macrophages: existence of different adhesive mechanisms and modulation by metabolic inhibitors.巨噬细胞的非特异性结合：不同黏附机制的存在及代谢抑制剂的调节作用
Immunology. 1980 Nov;41(3):547-60.
2
1-[4-(Trimethylamino)phenyl]-6-phenylhexa-1,3,5-triene: synthesis, fluorescence properties, and use as a fluorescence probe of lipid bilayers.1-[4-(三甲氨基)苯基]-6-苯基-1,3,5-己三烯：合成、荧光性质及其作为脂质双层荧光探针的应用
Biochemistry. 1981 Dec 22;20(26):7333-8. doi: 10.1021/bi00529a002.
3
TMA-DPH: a suitable fluorescence polarization probe for specific plasma membrane fluidity studies in intact living cells.TMA-DPH：一种适用于完整活细胞中特定质膜流动性研究的荧光偏振探针。
Cell Biophys. 1983 Jun;5(2):129-40. doi: 10.1007/BF02796139.
4
Rat epiphyseal cartilage. V. Glucose-C14 metabolism as related to growth and to various anatomical areas, in vitro.大鼠骨骺软骨。V. 体外葡萄糖 - C14代谢与生长及不同解剖区域的关系
Proc Soc Exp Biol Med. 1967 Feb;124(2):386-91. doi: 10.3181/00379727-124-31747.
5
Plasma membrane fluidity measurements on whole living cells by fluorescence anisotropy of trimethylammoniumdiphenylhexatriene.通过三甲基铵二苯基己三烯的荧光各向异性对完整活细胞进行质膜流动性测量。
Biochim Biophys Acta. 1985 Apr 22;845(1):60-7. doi: 10.1016/0167-4889(85)90055-2.
6
Parallel investigation of exocytosis kinetics and membrane fluidity changes in human platelets with the fluorescent probe, trimethylammonio-diphenylhexatriene.使用荧光探针三甲基铵-二苯基己三烯对人血小板胞吐动力学和膜流动性变化进行平行研究。
Biochim Biophys Acta. 1987 Jul 10;901(1):138-46. doi: 10.1016/0005-2736(87)90265-3.
7
A new simple fluorometric assay for phagocytosis.一种用于吞噬作用的新型简易荧光测定法。
J Immunol Methods. 1986 Apr 17;88(2):175-83. doi: 10.1016/0022-1759(86)90004-9.
8
Membrane fluidity changes in P. berghei-infected erythrocytes, investigated with a specific plasma membrane fluorescent probe.使用特定的质膜荧光探针研究伯氏疟原虫感染红细胞中的膜流动性变化。
Biochem Int. 1986 Jan;12(1):21-31.
9
Labelling of the inner side of granules membrane during exocytosis. A method to differentiate secretion processes.胞吐过程中颗粒膜内侧的标记。一种区分分泌过程的方法。
Agents Actions. 1986 Apr;18(1-2):53-6. doi: 10.1007/BF01987981.
10
A fluorescent hydrophobic probe used for monitoring the kinetics of exocytosis phenomena.一种用于监测胞吐现象动力学的荧光疏水探针。
Biochemistry. 1986 Apr 22;25(8):2149-54. doi: 10.1021/bi00356a045.

TMA-DPH a fluorescent probe of membrane dynamics in living cells. How to use it in phagocytosis.

作者信息

Illinger D, Kubina M, Duportail G, Poindron P, Bartholeyns J, Kuhry J G

机构信息

Département d' Immunologie, UA 491 du CNRS, Université Louis Pasteur, Strasbourg, France.

出版信息

Cell Biophys. 1989 Feb;14(1):17-26. doi: 10.1007/BF02797388.

DOI:10.1007/BF02797388
PMID:2465083
Abstract

Trimethylammonium-diphenylhexatriene (TMA-DPH), a hydrophobic fluorescent probe, has been shown in earlier studies to possess a variety of particular properties in interaction with intact living cells--specific and rapid incorporation into the plasma membrane and partition equilibrium between the membranes and the buffer. These properties offer promising applications in membrane fluidity studies and in monitoring exocytosis kinetics. Furthermore, these properties offer a method described here for quantitative monitoring of phagocytosis kinetics, by means of simple fluorescence intensity measurements. This method is original in that it evaluates only the particles which have actually been internalized by phagocytosis, and not those adsorbed on the cell surface, and that it gives quantitative information on the amount of plasma membrane involved in the process. It has been tested on mouse bone marrow macrophages.

摘要