Bronner C, Kuhry J G, Landry Y
Agents Actions. 1986 Apr;18(1-2):53-6. doi: 10.1007/BF01987981.
A fluorescence method to monitor quantitatively exocytosis phenomena in suspensions of intact cells was investigated, on the basis of the particular incorporation properties of a specific plasma membrane fluorescent label trimethylammonium-diphenylhexatriene (TMA-DPH). The method was tested with purified peritoneal mast cells stimulated by compound 48/80. Kinetics and dose-dependent response to compound 48/80 could be described accurately by the TMA-DPH fluorescence increase accompanying the exocytosis phenomenon, as controlled by conventional titrations of the released histamine. TMA-DPH bound to the outer side of the plasma membrane of resting cells and additively to the inner side of granules connected by pores to the exterior of stimulated mast cells.
基于特定质膜荧光标记三甲基铵 - 二苯基己三烯（TMA - DPH）的特殊掺入特性，研究了一种用于定量监测完整细胞悬液中胞吐现象的荧光方法。该方法用化合物48/80刺激的纯化腹膜肥大细胞进行了测试。伴随着胞吐现象的TMA - DPH荧光增加能够准确描述对化合物48/80的动力学和剂量依赖性反应，这通过对释放的组胺进行常规滴定来控制。TMA - DPH结合到静息细胞质膜的外侧，并额外结合到通过孔与受刺激肥大细胞外部相连的颗粒内侧。
